Department of Hematology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Leukemia. 2010 Mar;24(3):521-35. doi: 10.1038/leu.2009.268. Epub 2009 Dec 24.
Assessment of minimal residual disease (MRD) has acquired a prominent position in European treatment protocols for patients with acute lymphoblastic leukemia (ALL), on the basis of its high prognostic value for predicting outcome and the possibilities for implementation of MRD diagnostics in treatment stratification. Therefore, there is an increasing need for standardization of methodologies and harmonization of terminology. For this purpose, a panel of representatives of all major European study groups on childhood and adult ALL and of international experts on PCR- and flow cytometry-based MRD assessment was built in the context of the Second International Symposium on MRD assessment in Kiel, Germany, 18-20 September 2008. The panel summarized the current state of MRD diagnostics in ALL and developed recommendations on the minimal technical requirements that should be fulfilled before implementation of MRD diagnostics into clinical trials. Finally, a common terminology for a standard description of MRD response and monitoring was established defining the terms 'complete MRD response', 'MRD persistence' and 'MRD reappearance'. The proposed MRD terminology may allow a refined and standardized assessment of response to treatment in adult and childhood ALL, and provides a sound basis for the comparison of MRD results between different treatment protocols.
微小残留病灶（MRD）的评估在欧洲急性淋巴细胞白血病（ALL）治疗方案中占据重要地位，这是基于其对预后的高预测价值，以及在治疗分层中实施 MRD 诊断的可能性。因此，标准化方法和术语协调的需求日益增加。为此，在德国基尔举行的第二次 MRD 评估国际研讨会上，建立了一个由所有主要的欧洲儿童和成人 ALL 研究组的代表以及基于聚合酶链反应和流式细胞术的 MRD 评估国际专家组成的专家组。该小组总结了目前 ALL 中 MRD 诊断的现状，并就实施 MRD 诊断进入临床试验前应满足的最低技术要求提出了建议。最后，为了对 MRD 反应和监测进行标准描述，建立了一个通用术语，定义了“完全 MRD 反应”、“MRD 持续存在”和“MRD 再现”这三个术语。拟议的 MRD 术语可以对成人和儿童 ALL 的治疗反应进行精细化和标准化评估，并为不同治疗方案之间的 MRD 结果比较提供可靠基础。
