Dobritsa Anna A, Nishikawa Shuh-Ichi, Preuss Daphne, Urbanczyk-Wochniak Ewa, Sumner Lloyd W, Hammond Adam, Carlson Ann L, Swanson Robert J
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
Sex Plant Reprod. 2009 Sep;22(3):167-77. doi: 10.1007/s00497-009-0101-8. Epub 2009 Jun 11.
We isolated lap3-1 and lap3-2 mutants in a screen for pollen that displays abnormal stigma binding. Unlike wild-type pollen, lap3-1 and lap3-2 pollen exine is thinner, weaker, and is missing some connections between their roof-like tectum structures. We describe the mapping and identification of LAP3 as a novel gene that contains a repetitive motif found in beta-propeller enzymes. Insertion mutations in LAP3 lead to male sterility. To investigate possible roles for LAP3 in pollen development, we assayed the metabolite profile of anther tissues containing developing pollen grains and found that the lap3-2 defect leads to a broad range of metabolic changes. The largest changes were seen in levels of a straight-chain hydrocarbon nonacosane and in naringenin chalcone, an obligate compound in the flavonoid biosynthesis pathway.
我们在一项筛选显示异常柱头结合的花粉的实验中分离出了lap3-1和lap3-2突变体。与野生型花粉不同，lap3-1和lap3-2花粉的外壁更薄、更脆弱，并且其屋顶状覆盖层结构之间的一些连接缺失。我们描述了LAP3的定位和鉴定，它是一个含有在β-螺旋桨酶中发现的重复基序的新基因。LAP3中的插入突变导致雄性不育。为了研究LAP3在花粉发育中的可能作用，我们分析了含有发育中的花粉粒的花药组织的代谢物谱，发现lap3-2缺陷导致了广泛的代谢变化。最大的变化出现在直链烃二十九烷和柚皮素查耳酮（类黄酮生物合成途径中的一种必需化合物）的水平上。
