Back Jonathan, Scarpellino Léonardo, Held Werner
Ludwig Institute for Cancer Research Ltd., Lausanne Branch, and University of Lausanne, Epalinges, Switzerland.
Methods Mol Biol. 2010;612:313-23. doi: 10.1007/978-1-60761-362-6_21.
Certain receptors on natural killer (NK) cells, which are specific for MHC class I (MHC-I) molecules, do not only interact with ligand expressed on opposing cell membranes (in trans) but also interact with those on the same cell membrane (in cis). Cis interactions have been demonstrated for only a small number of cell surface receptors. However, this has not been tested systematically, raising the possibility that additional receptors may be able to bind ligand expressed in cis. Here we describe a number of approaches to evaluate trans and cis binding of the Ly49A NK cell receptor to its H-2D(d) ligand. These procedures should facilitate the investigation of cis/trans interactions of other receptor-ligand pairs and simplify the analysis of NK cell receptor variants.
自然杀伤（NK）细胞上的某些受体对主要组织相容性复合体I类（MHC-I）分子具有特异性，它们不仅与相对细胞膜上表达的配体（反式）相互作用，还与同一细胞膜上的配体（顺式）相互作用。仅少数细胞表面受体已被证实存在顺式相互作用。然而，尚未对此进行系统测试，这增加了其他受体可能能够结合顺式表达的配体的可能性。在此，我们描述了一些评估Ly49A NK细胞受体与其H-2D(d)配体的反式和顺式结合的方法。这些程序应有助于研究其他受体-配体对的顺式/反式相互作用，并简化NK细胞受体变体的分析。
