Simple and sensitive LC-ESI-MS method for the quantitation of sildenafil in plasma samples.

Sildenafil is a selective inhibitor of phosphodiesterase type 5 enzyme. A simple and sensitive LC-MS assay was developed to determine the concentration of sildenafil in human plasma. Sildenafil and omeprazole (internal standard) were extracted from the plasma with diethyl ether. The extract was evaporated under nitrogen and the residue was constituted with ACN and injected onto Novapak C(18) column (75x3.9 mm, 4 microm). The mobile phase consisted of 90% ACN plus 10% ammonium acetate (20 mM) containing 0.02% formic acid and was delivered isocratically at a flow rate of 0.2 mL/min. Sildenafil and omeprazole were monitored using a positive electrospray mode with single-ion recording set at m/z 475 and 346, respectively, which are consistent with M+H molecular ions, and the run time was less than 5 min. The detection limit of sildenafil was 0.5 ng/mL, and the calibration curve was linear between 0.5 and 2000 ng/mL (R(2)>0.99). Within- and between-day coefficients of variation were less than 7%. This method has been successfully used to measure sildenafil plasma concentrations in a beagle dog model following an oral administration of the drug.