Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Freiburg, Germany.
Dev Dyn. 2010 Feb;239(2):680-7. doi: 10.1002/dvdy.22194.
Anthrax Toxin Receptor 1 (ANTXR1; also known as Tumor Endothelial Marker 8, TEM8) is one of several genes that was recently found to be up-regulated in tumor-associated endothelial cells. In vitro, the protein can link extracellular matrix components with the actin cytoskeleton to promote cell adhesion and cell spreading. Both, ANTXR1 and the closely related ANTXR2 can bind anthrax toxin and interact with lipoprotein receptor-related protein 5 and 6, which also work as coreceptors in the WNT signaling pathway. Here, we report the cloning of chick ANTXR1 from a suppression subtractive hybridization screen for fibroblast growth factor (FGF) -inducible genes in chicken embryonic facial mesenchyme. We show that chicken ANTXR1 is dynamically expressed throughout embryogenesis, starting from Hamburger and Hamilton stage 10. Furthermore, we demonstrate that FGF signaling is sufficient, but not necessary, to induce ANTXR1 expression in chicken facial mesenchyme.
炭疽毒素受体 1（ANTXR1；也称为肿瘤内皮标记物 8，TEM8）是最近在肿瘤相关内皮细胞中发现上调的几个基因之一。在体外，该蛋白可以将细胞外基质成分与肌动蛋白细胞骨架连接起来，促进细胞黏附和细胞铺展。ANTXR1 和密切相关的 ANTXR2 都可以结合炭疽毒素，并与脂蛋白受体相关蛋白 5 和 6 相互作用，后者也作为 WNT 信号通路中的核心受体发挥作用。在这里，我们从鸡胚胎面部间充质中对成纤维细胞生长因子（FGF）诱导基因的抑制性消减杂交筛选中克隆了鸡 ANTXR1。我们表明，鸡 ANTXR1 在整个胚胎发生过程中动态表达，从 Hamburger 和 Hamilton 阶段 10 开始。此外，我们证明 FGF 信号足以诱导鸡面部间充质中 ANTXR1 的表达，但不是必需的。
