Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
Macromol Biosci. 2010 Mar 10;10(3):299-306. doi: 10.1002/mabi.200900223.
Insulin transport across the epithelial cell layer in the small intestine was studied using insulin/transferrin conjugates with and without the presence of P(MAA-g-EG) microparticles in contact with a co-culture of Caco-2/HT29-MTX cells. The insulin/transferrin conjugate was shown to increase transport relative to pure insulin by a factor of 7, achieving an apparent permeability of 37 x 10(9) cm . s(-1). The presence of P(MAA-g-EG) microparticles increased conjugate transport by a factor of 14 times relative to insulin, achieving an apparent permeability of 72.8 x 10(9) cm . s(-1). The presence of the microparticles in solution was found to improve conjugate transport by nearly 100% with little to no change in cell monolayer integrity.
采用胰岛素/转铁蛋白缀合物,研究了在共培养的 Caco-2/HT29-MTX 细胞中,有无 P(MAA-g-EG) 微粒存在的情况下,胰岛素在小肠上皮细胞层中的跨细胞转运。与纯胰岛素相比,胰岛素/转铁蛋白缀合物的转运增加了 7 倍,表观渗透系数达到 37×10(9)cm·s(-1)。存在 P(MAA-g-EG) 微粒使缀合物的转运增加了 14 倍,表观渗透系数达到 72.8×10(9)cm·s(-1)。发现微粒在溶液中的存在可使缀合物的转运提高近 100%,而对细胞单层完整性几乎没有影响。