Balimane Praveen V, Chong Saeho
Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb, Princeton, NJ 08543, USA.
Drug Discov Today. 2005 Mar 1;10(5):335-43. doi: 10.1016/S1359-6446(04)03354-9.
The model systems that are currently used to determine the intestinal permeability characteristics of discovery compounds often represent a combination of high-throughout, but less predictive, in silico and in vitro models and low-throughput, but more predictive, in vivo models. Cell-based permeability models have been integrated into the discovery paradigm for some time and represent the "method of choice" across the industry. Here, in addition to an objective analysis of the utility of cell culture models for permeability screening, anticipated future trends in the field of cell culture models are discussed.
目前用于确定研发化合物肠道通透性特征的模型系统通常是高通量但预测性较低的计算机模拟和体外模型与低通量但预测性较高的体内模型的组合。基于细胞的通透性模型已融入研发模式一段时间，是整个行业的“首选方法”。本文除了对细胞培养模型用于通透性筛选的效用进行客观分析外，还讨论了细胞培养模型领域预期的未来趋势。
