Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
Anal Biochem. 2010 Apr 15;399(2):225-9. doi: 10.1016/j.ab.2009.12.026. Epub 2009 Dec 23.
The ubiquitin-proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle. We demonstrate that the specific proteasomal activity differs in individual muscle groups and decreases with aging. These findings warrant the use of appropriate controls and a careful interpretation of results in mammalian skeletal muscle pathologies.
泛素-蛋白酶体系统 (UPS) 是一种主要的调节和错误折叠蛋白的降解系统。UPS 功能已被牵连在各种人类疾病的发病机制中发挥核心作用。由于生化分析常常受到可用疾病组织量的限制，我们报告了一种基于发光的蛋白酶体活性测定法的建立和验证，该方法适用于 5 毫克骨骼肌。我们证明，特定的蛋白酶体活性在不同的肌肉群中有所不同，并随着年龄的增长而降低。这些发现证明在哺乳动物骨骼肌病理学中使用适当的对照和仔细解释结果是必要的。
