Department of Biology, Brandon University, Brandon, Man. R7A 6A9, Canada.
Exp Parasitol. 2010 Apr;124(4):394-402. doi: 10.1016/j.exppara.2009.12.003. Epub 2009 Dec 23.
The effect of ivermectin and moxidectin on the motility of Brugia malayi adults and microfilariae and on the fertility of B. malayi females was examined. Motility was reduced in adults after exposure to both drugs and worms were non-motile and dead within eight days. The motility of microfilariae was significantly reduced at all drug concentrations and ceased at concentrations of 2500 and 5000mug/mL. The motility of microfilariae released by females was reduced after exposure to both drugs, however ivermectin had a greater effect at concentrations between 170 and 5000mug/mL. Both drugs reduced the number of microfilariae released by females and within four days their release was inhibited. The presence of the bacterial endosymbiont Wolbachia was examined in adults and microfilariae after exposure to increasing concentrations of ivermectin and moxidectin. A decrease in wsp expression was correlated with increasing drug concentration.
本研究旨在考察伊维菌素和莫昔克丁对马来丝虫成虫和微丝蚴的运动能力以及雌虫生殖能力的影响。两种药物均能显著降低成虫的运动能力，用药 8 天后虫体完全丧失运动能力并死亡。微丝蚴的运动能力在所有药物浓度下均显著降低，当药物浓度达到 2500 和 5000μg/ml 时，微丝蚴完全停止运动。两种药物均可降低雌虫释放的微丝蚴的运动能力，且伊维菌素的作用效果更为显著，在浓度为 170-5000μg/ml 时作用最强。两种药物均可减少雌虫释放的微丝蚴数量，用药 4 天后即可完全抑制微丝蚴释放。本研究还检测了伊维菌素和莫昔克丁在不同浓度下对成虫和微丝蚴中内共生菌沃尔巴克氏体的影响，结果发现 wsp 基因的表达与药物浓度呈负相关。
