Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500 007, India.
Fungal Genet Biol. 2010 Mar;47(3):237-45. doi: 10.1016/j.fgb.2009.12.006. Epub 2009 Dec 23.
Transcriptional analysis using qRT-PCR of 62 metal ion transporters during conidial germination of Neurospora crassa showed a significant up regulation of a hypothetical copper transporter gene, tcu-1, that belongs to the Ctr family. Herein we characterised the Ctr family genes (tcu-1, tcu-2 and tcu-3) and deciphered their role in various developmental phases of the N. crassa life cycle. Cross complementation assays in copper uptake mutant of Saccharomyces cerevisiae revealed that tcu-1, tcu-2 and tcu-3 are functional homologs of S. cerevisiae copper transporters. Expression studies of Ctr family members in various developmental phases of N. crassa showed differential expression pattern for high-affinity copper transporter, TCU1. Functional analysis of their gene knockout mutants showed that tcu-1 is essential for saprophytic conidial germination, vegetative growth and perithecia development under copper limited conditions while conidiation remained unaffected.
使用 qRT-PCR 对 Neurospora crassa 分生孢子萌发过程中的 62 种金属离子转运蛋白进行转录分析，显示假定铜转运蛋白基因 tcu-1 显著上调，该基因属于 Ctr 家族。在此，我们对 Ctr 家族基因（tcu-1、tcu-2 和 tcu-3）进行了特征描述，并阐明了它们在 N. crassa 生命周期的各个发育阶段中的作用。在铜摄取突变酵母 Saccharomyces cerevisiae 的交叉互补测定中，发现 tcu-1、tcu-2 和 tcu-3 是 S. cerevisiae 铜转运蛋白的功能同源物。在 N. crassa 的各个发育阶段对 Ctr 家族成员的表达研究表明，高亲和力铜转运蛋白 TCU1 的表达模式存在差异。它们的基因敲除突变体的功能分析表明，tcu-1 在铜限制条件下对腐生性分生孢子萌发、营养生长和产孢体发育是必需的，而分生孢子形成不受影响。
