编码锌毒性抑制因子的ZRC1的表达在酿酒酵母中以依赖Zap1的方式由锌饥饿胁迫诱导产生。

Expression of ZRC1 coding for suppressor of zinc toxicity is induced by zinc-starvation stress in Zap1-dependent fashion in Saccharomyces cerevisiae.

作者信息

Miyabe S, Izawa S, Inoue Y

机构信息

Research Institute for Food Science, Kyoto, 611-0011, Japan.

出版信息

Biochem Biophys Res Commun. 2000 Oct 5;276(3):879-84. doi: 10.1006/bbrc.2000.3580.

DOI:10.1006/bbrc.2000.3580

PMID:11027563

Abstract

The ZRC1 gene was cloned as a multicopy suppressor of zinc toxicity in Saccharomyces cerevisiae. Zrc1 belongs to CDF (cation diffusion facilitator) family. The transporters belonging to this family are thought to play an important role in metal detoxification. However, we found that cell growth of zrc1Delta mutant was lowered under the metal-limited conditions, which was restored by zinc. The Zap1 transcription factor is crucial for expression of several genes responsive to zinc-starvation stress. The expression of ZRC1 was induced in Zap1-dependent fashion when the intracellular zinc level was decreased and this induction was repressed by zinc. These results imply an important role of Zrc1 in the zinc-starvation stress.

摘要

ZRC1基因作为酿酒酵母中锌毒性的多拷贝抑制因子被克隆出来。Zrc1属于CDF（阳离子扩散促进因子）家族。该家族的转运蛋白被认为在金属解毒中起重要作用。然而，我们发现zrc1Delta突变体在金属限制条件下细胞生长降低，而锌可使其恢复。Zap1转录因子对于几个响应锌饥饿胁迫的基因的表达至关重要。当细胞内锌水平降低时，ZRC1的表达以依赖Zap1的方式被诱导，并且这种诱导被锌抑制。这些结果暗示Zrc1在锌饥饿胁迫中起重要作用。