Department of Molecular Parasitology, Humboldt University Berlin, Germany.
Microbes Infect. 2010 Mar;12(3):218-26. doi: 10.1016/j.micinf.2009.12.005. Epub 2009 Dec 23.
We investigated cellular immune responses of mice infected with the apicomplexan parasite Eimeria falciformis in order to characterise protective immune mechanisms and effector functions. Adoptive transfer experiments with mesenterial lymph node cells (MLNC) from immune donor mice were performed, and the oocyst output monitored after challenge infection. Phenotypical analysis by fluorescence cytometry and T cell proliferation assay showed that already from day four post infection E. falciformis-specific lymphocytes were present in the MLN. The frequency of parasite-specific, IFN-gamma producing CD4+ and CD8+ cells increased in this period by 9.8% and 16.4%, respectively. Infection experiments with IFN-gamma deficient mice revealed that IFN-gamma is involved in resistance to primary and secondary infection. Transfer of total MLNC from immune donors reduced the oocyst output by 65-74%, as compared to the oocyst output of animals transferred with cells from naïve donors. Transfer of CD8+ cells inhibited parasite development resulting in a reduction of oocyst numbers by 42-64%, whereas CD4+ cells showed no influence on resistance to reinfection.
我们研究了感染顶复门原虫新月形艾美耳球虫的小鼠的细胞免疫反应，以阐明保护性免疫机制和效应功能。我们进行了肠系膜淋巴结细胞（MLNC）的过继转移实验，并用免疫供体小鼠进行实验，并在挑战感染后监测卵囊的产量。通过荧光细胞术和 T 细胞增殖试验进行表型分析表明，从感染后第四天开始，MLN 中就存在针对新月形艾美耳球虫的特异性淋巴细胞。在此期间，寄生虫特异性 IFN-γ产生的 CD4+和 CD8+细胞的频率分别增加了 9.8%和 16.4%。用 IFN-γ缺陷型小鼠进行的感染实验表明，IFN-γ参与了对初次和再次感染的抵抗。与从幼稚供体转移的细胞相比，从免疫供体转移的总 MLNC 可将卵囊产量降低 65-74%。转移 CD8+细胞抑制寄生虫的发育，导致卵囊数量减少 42-64%，而 CD4+细胞对再次感染的抵抗力没有影响。
