[广州石蜡包埋宫颈癌样本中致癌型人乳头瘤病毒的鉴定与基因分型]
[Identification and genotyping of oncogenic type of human papillomavirus in paraffin-embedded cervical cancer samples in Guangzhou].
作者信息
Zeng Li, Yu Shou-yi, Gou Sui-qun, Yun Jin-ping, Zhang Jun
机构信息
Department of Epidemiology, School of Public Health and Tropical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Dec;29(12):2485-7.
OBJECTIVE
To investigate human papillomavirus (HPV) infection and genotyping in patients with cervical cancer in Guangzhou in the last 3 decades.
METHODS
HPV L1 gene fragment in paraffin-embedded cervical cancer samples was amplified by HPV-specific PCR with consensus primers, and typing of HPV strains was performed on the basis of sequence analysis of the PCR products.
RESULTS
The positivity rates of HPV DNA was 26.2% in the 99 cases of cervical cancer. Five HPV genotypes were identified including HPV16, 18, 33, 52 and 58.
CONCLUSION
HPV16, 58 and 33 are the most common genotypes of HPV, accounting for over 88.4% in the total infected cases, suggesting that the HPV genotypes closely related to cervical cancer is more centralized in Guangzhou.
目的
调查过去30年广州宫颈癌患者人乳头瘤病毒（HPV）感染及基因分型情况。
方法
采用通用引物通过HPV特异性PCR扩增石蜡包埋宫颈癌样本中的HPV L1基因片段，并基于PCR产物的序列分析进行HPV毒株基因分型。
结果
99例宫颈癌患者中HPV DNA阳性率为26.2%。鉴定出5种HPV基因型，包括HPV16、18、33、52和58。
结论
HPV16、58和33是最常见的HPV基因型，占总感染病例的88.4%以上，表明广州与宫颈癌密切相关的HPV基因型更为集中。
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