[采用聚合酶链反应和直接测序法检测宫颈癌组织中的人乳头瘤病毒DNA]
[Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing].
作者信息
Liu Xin, Liu Guo-Bing, Pang Zhan-Jun, Xing Fu-Qi, Guo Sui-Qun, Ma Wen-Li, Zheng Wen-Ling
机构信息
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2005 Feb;25(2):201-3.
OBJECTIVE
To explore an effective method for detecting human papillomavirus (HPV) DNA in cervical cancer tissue.
METHODS
HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product.
RESULTS
The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections. Infection with HPV58 was detected in one case. The sequencing results showed no difference in L1 sequence between the detected samples and the standard German HPV58 strain.
CONCLUSION
PCR and direct sequencing approach is effective for detecting and typing of HPV DNA in cervical cancer tissue, through which rare HPV strain or mutants of known HPV strains may not escape detection.
目的
探索一种检测宫颈癌组织中人乳头瘤病毒（HPV）DNA的有效方法。
方法
采用通用引物通过HPV特异性PCR扩增宫颈癌组织中的HPV L1基因片段，并根据PCR产物的序列分析对HPV毒株进行分型。
结果
50例宫颈癌患者中HPV DNA阳性率为78%，HPV16和HPV18毒株混合感染最为常见，占总感染数的48%。检测到1例HPV58感染。测序结果显示，检测样本与标准德国HPV58毒株的L1序列无差异。
结论
PCR和直接测序方法可有效检测宫颈癌组织中的HPV DNA并进行分型，通过该方法罕见的HPV毒株或已知HPV毒株的突变体可能无法逃脱检测。
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