稳定期 COPD 患者外周血 T 细胞谱与临床和炎症参数的相关性。

Correlation between peripheral blood T-cell profiles and clinical and inflammatory parameters in stable COPD.

机构信息

Department of Respiratory Medicine, Shizuoka General Hospital, and Hamamatsu University School of Medicine, Shizuoka, Japan.

出版信息

Allergol Int. 2010 Mar;59(1):75-82. doi: 10.2332/allergolint.09-OA-0126. Epub 2009 Dec 25.

DOI:10.2332/allergolint.09-OA-0126

PMID:20035145

Abstract

BACKGROUND

Recent studies suggest that Tc1/Tc2 imbalances are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The purpose of this study was to clarify the relationship between peripheral blood T-cell profiles and pulmonary function or inflammatory parameters.

METHODS

Thirty-one patients with stable COPD (median age 70 years, 30 males, 15 current smokers and 16 ex-smokers) and 30 healthy control subjects were enrolled in this study. The subjects underwent blood tests, exhaled nitric oxide (eNO) measurement, pulmonary function tests, and sputum induction. Tc1/Tc2 and Th1/Th2 were determined by analyzing intracellular cytokine staining for IFN-gamma and IL-4 in peripheral blood CD8+ and CD4+ T cells using flow cytometry after stimulation with phorbol 12-myristate 13-acetate and ionomycin.

RESULTS

There was a significantly increased proportion of IFN-gamma-producing and IL-4-producing CD8+ T cells in patients with COPD compared with control subjects (median [IQR] 73.6% [63.9%-80.7%] vs 62.0% [45.6%-73.8%], p=0.004; and 2.6% [1.1%-6.9%] vs 1.1% [0.6%-2.2%], p=0.002, respectively). In addition, the proportion of IFN-gamma-producing CD4+ T cells was significantly higher in patients with COPD compared with control subjects (25.7% [21.2%-38.0%] vs 22.8% [15.6%-29.2%], p=0.027). The proportion of IFN-gamma-producing CD8+ T cells was correlated negatively with single-breath carbon monoxide transfer coefficient (Kco)(rho=-0.45, p=0.033) and positively with eNO (rho=0.50, p=0.012). The proportion of IL-4-producing CD8+ T cells was positively correlated with body mass index (rho=0.42, p=0.023) and Kco (rho=0.47, p=0.026).

CONCLUSIONS

It is suggested that Tc1 cells have a detrimental role and that Tc2 cells have a protective role in disease progression.

摘要

背景

最近的研究表明，Tc1/Tc2 失衡与慢性阻塞性肺疾病（COPD）的发病机制有关。本研究的目的是阐明外周血 T 细胞谱与肺功能或炎症参数之间的关系。

方法

纳入 31 例稳定期 COPD 患者（中位年龄 70 岁，30 名男性，15 名现吸烟者和 16 名曾吸烟者）和 30 名健康对照者。对受试者进行血液检查、呼出气一氧化氮（eNO）测量、肺功能检查和痰诱导。通过分析 PMA 和离子霉素刺激后外周血 CD8+和 CD4+T 细胞中 IFN-γ和 IL-4 的细胞内细胞因子染色，使用流式细胞术确定 Tc1/Tc2 和 Th1/Th2。

结果

与对照组相比，COPD 患者 IFN-γ产生和 IL-4 产生的 CD8+T 细胞比例显著增加（中位数[IQR]73.6%[63.9%-80.7%] vs 62.0%[45.6%-73.8%]，p=0.004；2.6%[1.1%-6.9%] vs 1.1%[0.6%-2.2%]，p=0.002）。此外，COPD 患者 IFN-γ产生的 CD4+T 细胞比例也明显高于对照组（25.7%[21.2%-38.0%] vs 22.8%[15.6%-29.2%]，p=0.027）。IFN-γ产生的 CD8+T 细胞比例与单呼气一氧化碳传递系数（Kco）呈负相关（rho=-0.45，p=0.033），与 eNO 呈正相关（rho=0.50，p=0.012）。IL-4 产生的 CD8+T 细胞比例与体重指数（rho=0.42，p=0.023）和 Kco（rho=0.47，p=0.026）呈正相关。