Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, 500 046, India.
Fish Physiol Biochem. 2005 Apr;31(2-3):157-61. doi: 10.1007/s10695-006-0019-0.
Vertebrate reproduction is under the neuroendocrine control of the hypothalamic decapeptide GnRH which synchronizes various reproductive events and influences other reproduction related aspects like spawning behavior and pheromonal action in fish. Multiple forms of GnRH peptides have been reported across diverse vertebrate and invertebrate classes. Here we report the partial seabream GnRH (sbGnRH) cDNA sequence cloned from the brain of Channa striatus (snake head murrel) a fresh water perciform with immense economic and medicinal value across Asiatic countries. sbGnRH mRNA was found in brain, gill and ovary of mature murrel with possible implications to the effect of GnRH on pheromonal phenomena and on reinitiation of oocyte meiosis. In keeping with the earlier reported role of GnRH in initiation of oocyte meiosis we here present evidence from RT-PCR, ICC demonstrating an increase in the level of sbGnRH mRNA in ovary from pre-vitellogenic to post-vitellogenic follicles.
脊椎动物的繁殖受到下丘脑十肽 GnRH 的神经内分泌控制，它使各种生殖事件同步，并影响其他与生殖相关的方面，如鱼类的产卵行为和信息素作用。已经在不同的脊椎动物和无脊椎动物门中报告了多种 GnRH 肽形式。在这里，我们报告了从 Channa striatus（蛇头鲷）脑中克隆的部分半滑舌鳎 GnRH（sbGnRH）cDNA 序列，蛇头鲷是一种具有巨大经济和药用价值的亚洲淡水鲈形目鱼类。在成熟的鲷鱼的大脑、鳃和卵巢中发现了 sbGnRH mRNA，这可能暗示 GnRH 对信息素现象和卵母细胞减数分裂的重新启动有影响。与 GnRH 启动卵母细胞减数分裂的早期报道作用一致，我们在此通过 RT-PCR 和 ICC 提供证据，证明 sbGnRH mRNA 在从卵黄前滤泡到卵黄后滤泡的卵巢中的水平增加。
