Cytheris S.A., Technopolis, 175 rue J.J. Rousseau, 92130 Issy les Moulineaux, France.
J Immunol Methods. 2010 Feb 28;353(1-2):115-23. doi: 10.1016/j.jim.2009.12.003. Epub 2009 Dec 24.
IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is important to develop a sensitive and reliable assay in order to investigate the potential role of this receptor in the regulation of IL-7 physiologic, physipathologic and therapeutic effects. We here report a fully validated method to measure sCD127 in human and simian plasma using a method based on ELISA MSD technology. We demonstrate that sCD127 is detectable at various levels in the plasma of healthy humans as well as in that of healthy Rhesus and Cynomolgus macaques (106.72, 205.26 and 366.95 ng/mL respectively). Moreover, as opposed to the sCD25/IL-2 tandem, we demonstrate that IL-7 treatment has no impact on sCD127 plasma concentration in patients infected by HIV.
白细胞介素 7（IL-7）是 T 细胞造血和外周稳态的关键细胞因子，通过其受体α链（CD127）信号传导对于诱导 T 细胞增殖和存活至关重要。由于特异性 CD127α链以可溶形式（sCD127）和高浓度存在于血浆中（ng/mL），因此开发一种敏感和可靠的测定法以研究该受体在调节白细胞介素 7 的生理、病理生理和治疗作用方面的潜在作用非常重要。我们在此报告了一种使用基于 ELISA MSD 技术的方法来测量人源和灵长类动物血浆中 sCD127 的完全验证方法。我们证明 sCD127 在健康人类以及健康恒河猴和食蟹猴的血浆中可检测到不同水平（分别为 106.72、205.26 和 366.95ng/mL）。此外，与 sCD25/IL-2 串联物相反，我们证明 HIV 感染患者的白细胞介素 7 治疗对 sCD127 血浆浓度没有影响。
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