Department of Physics and Electronics Science, Chuxiong Normal University, Chuxiong 675000, China.
J Colloid Interface Sci. 2010 Mar 1;343(1):52-7. doi: 10.1016/j.jcis.2009.11.042. Epub 2009 Nov 23.
A new SERS-active Ag nanofilm on the surface of a glass slide has been prepared by a low-cost electrochemical strategy using polyvinyl alcohol (PVA) at a proper voltage. The two-dimensional morphology of the Ag nanofilm has been examined by scanning electron microscopy (SEM). The average size of the aggregated particles on the surface of the Ag nanofilm is up to ca. 200+/-50 nm, which is much larger than that of PVA-protected Ag colloidal nanoparticles (PVA-Ag CNPs, 45+/-8 nm). Meanwhile, many nano-scale regions with average sizes of ca. 300+/-50 nm are formed between the adjacent Ag nanoparticles. By the SERS measurements of human serum (HS) and hemoglobin (Hb), this Ag nanofilm is shown to be an excellent SERS substrate with good stability and biocompatibility. As the fabrication process of this SERS substrate is simple and inexpensive, this method may be used in large-scale preparation of substrates that have been widely applied in Raman analysis. In addition, this SERS-active Ag nanofilm can serve as a novel SERS substrate in biochemical analysis due to the biocompatibility.
一种新型的 SERS 活性 Ag 纳米薄膜通过使用聚乙烯醇（PVA）在适当的电压下的低成本电化学策略在玻璃载玻片表面制备。Ag 纳米薄膜的二维形貌通过扫描电子显微镜（SEM）进行了检查。Ag 纳米薄膜表面上聚集颗粒的平均尺寸高达约 200+/-50nm，远远大于 PVA 保护的 Ag 胶体纳米粒子（PVA-Ag CNPs，45+/-8nm）。同时，在相邻 Ag 纳米颗粒之间形成了许多具有平均尺寸约 300+/-50nm 的纳米级区域。通过对人血清（HS）和血红蛋白（Hb）的 SERS 测量，表明该 Ag 纳米薄膜是一种具有良好稳定性和生物相容性的优异 SERS 基底。由于这种 SERS 基底的制造工艺简单且廉价，因此该方法可用于广泛应用于拉曼分析的基底的大规模制备。此外，由于生物相容性，这种 SERS 活性 Ag 纳米薄膜可用作生化分析中的新型 SERS 基底。
