Biotecnología de la Reproducción, Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, C.C. 276, 7620 Balcarce, Argentina.
Anim Reprod Sci. 2010 May;119(1-2):1-8. doi: 10.1016/j.anireprosci.2009.11.009. Epub 2009 Dec 3.
This study was conducted to investigate in early postpartum suckled beef cows with and without FSH pre-stimulation: (i) the influence of the postpartum period on the number and quality of oocytes recovered by ovum pick-up (OPU), (ii) the overall efficiency of the OPU/IVP embryos from days 30 to 80 postpartum and (iii) if repeated OPU negatively affect fertility following a fixed-time artificial insemination protocol. After parturition suckled Angus cows (n = 30) were divided in three groups (n = 10 group(-1)). All cows were anestrous at the commencement of experimental treatments (30.0 +/- 3.2 days postpartum, mean +/- SD; range 25-34 days). Group 1 treatments included: dominant follicle ablation (DFA), FSH treatment and OPU procedure 5 days after DFA. A total of 9 mg FSH (Ovagen) was administered s.c. once a day over 2 days at equal doses (4.5 + 4.5mg). For fertility test the cows received an intravaginal progesterone treatment from Days 78 to 86 postpartum and were fixed-time artificially inseminated (FTAI) at 56 and 72 h after device removal. Group 2: as cited for Group 1 with no FSH treatment. In both groups, OPU was repeated four times (Days 35, 49, 63 and 77 postpartum) and the collected oocytes classified as viable were in vitro matured, fertilized and presumptive embryos cultured for 8 days. Group 3 (Control FTAI): cows that had not previously aspirations were FTAI as Groups 1 and 2. Pregnancy was diagnosed by means ultrasonography 39 days after FTAI. The numbers (mean +/- SEM) of follicles visible and aspirated at the time of OPU in FSH-treated cows were greater (P < 0.05) than in non-treated cows (10.6 +/- 0.6 and 8.4 +/- 0.4 vs. 8.0 +/- 0.5 and 4.6 +/- 0.3, respectively). Following FSH treatment, the number (mean +/- SEM) of recovered oocytes per cow per OPU session and percentage of viable oocytes were greater in the treated (P < 0.05) than in non-treated animals (3.0 +/- 0.1 and 39.5% vs. 1.5 +/- 0.1 and 30.0%). The cleavage and embryo development rates were similar (P > 0.05) for both groups (14.8 and 6.4% vs. 16.6 and 5.5%). After FTAI the pregnancy rates were not different (P > 0.05) among groups (70, 60 and 90% for Groups 1, 2 and 3, respectively). We can conclude that (1) FSH-treated suckled postpartum cows can be a source of oocytes for in vitro fertilization and (2) repeated DFA/OPU applied during postpartum period did not affect the subsequent fertility following FTAI.
本研究旨在探讨在产后哺乳期有和没有 FSH 预处理的情况下：（i）产后对通过卵母细胞抽吸（OPU）回收的卵母细胞数量和质量的影响，（ii）产后 30 至 80 天 OPU/IVP 胚胎的总体效率，以及（iii）如果重复 OPU 是否会对固定时间人工授精（FTAI）方案后的生育能力产生负面影响。产后哺乳期安格斯牛（n=30）分为三组（n=10 组）。所有奶牛在实验治疗开始时均处于乏情状态（30.0 +/- 3.2 天产后，平均值 +/- SD；范围 25-34 天）。第 1 组治疗包括：优势卵泡消融（DFA）、FSH 处理和 DFA 后 5 天进行 OPU 程序。共给予 9mg FSH（Ovagen），每天皮下注射一次，连续 2 天，剂量相等（4.5+4.5mg）。为了进行生育力测试，奶牛在产后第 78 至 86 天接受阴道内孕酮治疗，并在装置去除后 56 和 72 小时进行定时人工授精（FTAI）。第 2 组：与第 1 组相同，不进行 FSH 处理。在两组中，重复进行 4 次 OPU（产后第 35、49、63 和 77 天），并将收集的有活力的卵母细胞进行体外成熟、受精和推定胚胎培养 8 天。第 3 组（对照 FTAI）：未进行抽吸的奶牛与第 1 组和第 2 组进行 FTAI。FTAI 后 39 天通过超声检查诊断怀孕。FSH 处理组奶牛在 OPU 时可见和抽吸的卵泡数量（平均值 +/- SEM）大于未处理组（10.6 +/- 0.6 和 8.4 +/- 0.4 与 8.0 +/- 0.5 和 4.6 +/- 0.3 相比）。在 FSH 处理后，每头奶牛每次 OPU 回收的卵母细胞数量（平均值 +/- SEM）和有活力的卵母细胞百分比在处理组中较高（P<0.05），而非处理组（3.0 +/- 0.1 和 39.5% 与 1.5 +/- 0.1 和 30.0%）。两组的卵裂和胚胎发育率相似（P>0.05）（14.8%和 6.4% 与 16.6%和 5.5%）。FTAI 后，各组妊娠率无差异（P>0.05）（第 1、2 和 3 组分别为 70%、60%和 90%）。我们可以得出结论：（1）FSH 处理的哺乳期产后奶牛可以成为体外受精的卵母细胞来源，（2）产后期间重复进行 DFA/OPU 不会影响 FTAI 后的后续生育能力。
