Surjus Ricardo S, Prata Alexandre B, Borsato Marta, Mattos Fernanda C S Z, Martins da Silveira Mariana C, Mourão Gerson B, Pires Alexandre V, Wiltbank Milo C, Sartori Roberto
Department of Animal Science, College of Agriculture 'Luiz de Queiroz' (ESALQ), University of São Paulo, Av. Pádua Dias, 11, CP 9, Piracicaba, SP 13418-900, Brazil.
College of Veterinary and Animal Science, São Paulo State University, Distrito Rubião Júnior, Botucatu, SP 18618-970, Brazil.
Reprod Fertil Dev. 2014;26(4):527-32. doi: 10.1071/RD12398.
The present study evaluated superovulatory responses and in vivo embryo production in cows treated with FSH starting 1 or 2 days after ovum pick-up (OPU). Thirty-three non-lactating Nelore cows were subjected to aspiration of all follicles ≥3mm for OPU. After OPU, cows were randomly divided into two groups in which the follicle superstimulatory treatments with FSH started 1 or 2 days after OPU (Groups D1 and D2, respectively). Data are presented as the least squares mean±s.e.m. The number of follicles ≥3mm before OPU was similar between groups (~34); however, cows in Group D2 had more follicles ≥3mm on the first day of FSH (15.2±2.3 vs 7.6±1.7; P=0.04) and a higher ratio of the number of follicles at first FSH/number of follicles before OPU (0.41±0.04 vs 0.24±0.02; P=0.01). In addition, Group D2 cows had a greater superovulatory response than did cows in Group D1 (18.9±2.8 vs 9.1±1.9 corpora lutea, respectively; P<0.03). However, there was no difference in the total number of recovered ova and embryos from cows in Groups D2 and D1 (5.1±1.4 vs 4.9±1.3, respectively; P>0.10). Nevertheless Group D2 cows had more freezable embryos than Group D1 cows (3.2±1.1 vs 1.3±0.5, respectively; P<0.05). Cows from Group D2 had a much higher proportion (P<0.001) of follicles ≥8mm compared with follicles ≥6mm and <8mm at the time of the last treatment with FSH. In conclusion, to obtain a greater production of viable embryos in superovulated cows after OPU, it is recommended to wait at least 2 days before starting FSH treatment.
本研究评估了在采卵（OPU）后1天或2天开始用促卵泡素（FSH）处理的奶牛的超排卵反应及体内胚胎生产情况。33头非泌乳内罗牛接受了OPU，对所有直径≥3mm的卵泡进行抽吸。OPU后，奶牛被随机分为两组，分别在OPU后1天或2天开始用FSH进行卵泡超刺激处理（分别为D1组和D2组）。数据以最小二乘均值±标准误表示。OPU前直径≥3mm的卵泡数量在两组间相似（约34个）；然而，D2组奶牛在开始使用FSH的第一天有更多直径≥3mm的卵泡（15.2±2.3对7.6±1.7；P=0.04），且首次使用FSH时卵泡数量与OPU前卵泡数量的比值更高（0.41±0.04对0.24±0.02；P=0.01）。此外，D2组奶牛的超排卵反应比D1组奶牛更强（分别为18.9±2.8个黄体对9.1±1.9个黄体；P<0.03）。然而，D2组和D1组奶牛回收的卵子和胚胎总数没有差异（分别为5.1±1.4对4.9±1.3；P>0.10）。不过，D2组奶牛比D1组奶牛有更多可冷冻胚胎（分别为3.2±1.1对1.3±0.5；P<0.05）。在最后一次使用FSH时，D2组奶牛直径≥8mm的卵泡比例与直径≥6mm且<8mm的卵泡比例相比要高得多（P<0.001）。总之，为了在OPU后的超排奶牛中获得更多可存活胚胎，建议在开始FSH处理前至少等待2天。
