Xia Junxia, Qiao Fuyuan, Su Fangmin, Liu Haiyi
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2009 Dec;29(6):755-60. doi: 10.1007/s11596-009-0617-z. Epub 2009 Dec 29.
To investigate the expression of osteopontin (OPN) and its receptor integrin alphanubeta3 in the placental tissue from pregnant women complicated with preeclampsia, the expression of OPN and alphanubeta3 in the placenta of the pregnant women with preeclampsia and healthy pregnant women was detected by immunohistochemistry, Western blotting and RT-PCR. Our results showed that OPN and alphanubeta3 protein were expressed in the placenta from normal pregnant woman and those with preeclampsia. OPN was located in the placental syncytiotrophoblasts and the cytoplasm of capillary endothelial cells and integrin alphanubeta3 was mainly expressed on the surface of trophoblast cells. Expression of OPN and integrin alphanubeta3 in the placental tissue from preeclampsia subjects was significantly lower than that from the control group (P<0.05). Compared with the control group, expression of OPN in the placental tissue from preeclampsia group was significantly lower (P<0.05) but there was no significant difference in the expression of alphanu and beta3 between the preeclampsia group and the controls. It is concluded that OPN and its receptor integrin alphanubeta3 may be involved in the pathogenesis of preeclampsia.
为研究骨桥蛋白（OPN）及其受体整合素αvβ3在子痫前期孕妇胎盘组织中的表达情况，采用免疫组织化学、蛋白质印迹法及逆转录聚合酶链反应（RT-PCR）检测子痫前期孕妇及正常孕妇胎盘中OPN及αvβ3的表达。结果显示，正常孕妇及子痫前期孕妇胎盘中均有OPN及αvβ3蛋白表达。OPN定位于胎盘合体滋养层细胞及毛细血管内皮细胞胞质，整合素αvβ3主要表达于滋养层细胞表面。子痫前期患者胎盘组织中OPN及整合素αvβ3的表达明显低于对照组（P<0.05）。与对照组相比，子痫前期组胎盘组织中OPN表达明显降低（P<0.05），但子痫前期组与对照组之间αv及β3的表达无明显差异。结论：OPN及其受体整合素αvβ3可能参与子痫前期的发病机制。
