网膜切除术对细菌清除的影响：一项实验研究。

The influence of omentectomy on bacterial clearance: an experimental study.

作者信息

Uzunköy Ali, Ozbilge Hatice, Horoz Mehmet

机构信息

Department of General Surgery, Harran University, Faculty of Medicine, Sanliurfa, Turkey.

出版信息

Ulus Travma Acil Cerrahi Derg. 2009 Nov;15(6):541-5.

PMID:20037870

Abstract

BACKGROUND

The influence of an omentectomy on peritoneal defense mechanisms and its clinical consequences have not been fully elucidated. In the present study, we aimed to investigate the influence of omentectomy on bacterial growth in blood and tissue specimen cultures in rats with experimental peritonitis.

METHODS

Fifty Wistar-Albino rats were included in the present study. Animals were assigned into five groups as follow: Group 1 (n = 10), omentectomy alone; Group 2 (n = 10), omentectomy + bacterial peritonitis; Group 3 (n = 10), bacterial peritonitis alone; Group 4 (n = 10), laparotomy alone; and Group 5 (n = 10), sham group. Culture positivity rate and bacterial growth (colony forming units [CFU]/gram tissue) were assessed in mesenteric lymphoid tissue and venous blood of all animals.

RESULTS

Bacterial growth in lymphoid tissue was significantly higher in Groups 2 and 3 than others (both, p < 0.05). CFU of Escherichia coli in lymphoid tissue was significantly higher in Group 2 than in Groups 1 and 3 (both, p < 0.05/4). Blood culture positivity was significantly higher in Group 2 than the others (both, p < 0.05).

CONCLUSION

The omentum has an important role in the host peritoneal defense system. Peritoneal infection may pursue a more severe course with increased bacterial entrance into the blood in the absence of the omentum.

摘要

背景

大网膜切除术对腹膜防御机制的影响及其临床后果尚未完全阐明。在本研究中，我们旨在调查大网膜切除术对实验性腹膜炎大鼠血液和组织标本培养中细菌生长的影响。

方法

本研究纳入了50只Wistar白化大鼠。动物被分为五组，如下：第1组（n = 10），单纯大网膜切除术；第2组（n = 10），大网膜切除术 + 细菌性腹膜炎；第3组（n = 10），单纯细菌性腹膜炎；第4组（n = 10），单纯剖腹术；第5组（n = 10），假手术组。评估所有动物肠系膜淋巴组织和静脉血中的培养阳性率和细菌生长情况（菌落形成单位[CFU]/克组织）。