直接从牛粪便中提取 DNA 之前进行冻干可提高大肠杆菌 O157:H7 和空肠弯曲菌的定量检测。
Lyophilization prior to direct DNA extraction from bovine feces improves the quantification of Escherichia coli O157:H7 and Campylobacter jejuni.
机构信息
Climate, Land and Environment, AgResearch, East Street, Private Bag 3123, Hamilton 3240, New Zealand.
出版信息
Appl Environ Microbiol. 2010 Mar;76(5):1686-8. doi: 10.1128/AEM.01866-09. Epub 2009 Dec 28.
Abstract
Lyophilization was used to concentrate bovine feces prior to DNA extraction and analysis using real-time PCR. Lyophilization significantly improved the sensitivity of detection compared to that in fresh feces and was associated with reliable quantification of both Escherichia coli O157:H7 and Campylobacter jejuni bacteria present in feces at concentrations ranging between 2 log(10) and 6 log(10) CFU g(-)(1).
摘要
采用冷冻干燥法浓缩牛粪便中的 DNA 后，采用实时 PCR 进行分析。与新鲜粪便相比，冷冻干燥显著提高了检测的灵敏度，并且与粪便中浓度在 2 log(10) 至 6 log(10) CFU g(-)(1) 范围内的大肠杆菌 O157:H7 和空肠弯曲菌的可靠定量相关。
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