用于检测牛粪便样本中大肠杆菌O157的实时荧光定量PCR试剂盒的评估
Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples.
作者信息
Bono James L, Keen James E, Miller Laura C, Fox James M, Chitko-McKown Carol G, Heaton Michael P, Laegreid William W
机构信息
U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166, USA.
出版信息
Appl Environ Microbiol. 2004 Mar;70(3):1855-7. doi: 10.1128/AEM.70.3.1855-1857.2004.
Abstract
A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.
摘要
对一种市售的实时快速聚合酶链反应（PCR）检测方法检测大肠杆菌O157的能力进行了评估。对于纯培养物中的分离株，该检测方法的灵敏度和特异性均为99%。在富集后，该检测方法能检测出人工接种的牛粪中每克含1个大肠杆菌O157:H7菌落形成单位（CFU）。
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