Dr. Ondokuz Mayis University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Samsun, Turkey.
Med Oral Patol Oral Cir Bucal. 2010 May 1;15(3):e526-30.
The purpose of this study is to investigate the effects of monocryl (Polyglecaprone 25), silk and catgut suture materials on wound healing in diabetic rats and to evaluate NO (nitric oxide) dynamics.
Fourty-eight male Wistar-Albino rats weighing 220-270 g were used in this study. The rats were categorized into 2 groups, as control group (n=24) and streptozotocin (STZ) induced diabetic group (n=24). For each group, incision wounds were created on the inner cheek mucosa of the animals and wounds were closed primarily with three different types of sutures. These materials were sized 3-0 monocryl (polyglecaprone 25), silk and catgut sutures swaged on. Excisional biopsies were performed at 2nd, 7th, 14th and 21st day (2 rats per day) from the initial surgical procedure. Effects of these suture materials on wound healing and NOS (nitric oxide synthase) activities were compared immunohistochemically in the study. eNOS and iNOS (Endothelial and induced nitric oxide synthase) activities were classified as mild (+), moderate (++) and severe (+++).
Mild eNOS activities were seen in both diabetic and control groups at 2nd day specimens. Activities of silk and catgut were similar both in diabetic and control groups at 14th day, this activity in monocryl was mild. 21st day specimens revealed that monocryl has mild eNOS activity than silk and catgut sutures. iNOS activities in diabetic group were greater than control groups and the degrees of the activity in monocryl, silk and catgut sutures were mild, severe and moderate at the 7th day. At the 14th day there was no iNOS activity in diabetic monocryl group.
All of the materials have the potential capacity to be well tolerated however monocryl seemed to have more beneficial effects on wound healing in diabetic subjects.
本研究旨在探讨单丝（聚己内酯 25）、丝线和肠线缝线材料对糖尿病大鼠伤口愈合的影响，并评估一氧化氮（NO）动力学。
本研究使用了 48 只雄性 Wistar-Albino 大鼠，体重为 220-270 克。这些大鼠被分为 2 组，对照组（n=24）和链脲佐菌素（STZ）诱导的糖尿病组（n=24）。对于每组，在动物的内颊黏膜上创建切口伤口，并使用三种不同类型的缝线进行一期缝合。这些缝线材料为 3-0 单丝（聚己内酯 25）、丝线和肠线缝线。在初始手术 2、7、14 和 21 天后（每天 2 只大鼠）进行切除活检。在研究中，通过免疫组织化学比较这些缝线材料对伤口愈合和NOS（一氧化氮合酶）活性的影响。eNOS 和 iNOS（内皮和诱导型一氧化氮合酶）活性分为轻度（+）、中度（++）和重度（+++）。
在第 2 天标本中，糖尿病组和对照组均可见轻度 eNOS 活性。在第 14 天，丝线和肠线在糖尿病组和对照组中的活性相似，而单丝的活性为轻度。第 21 天标本显示，与丝线和肠线缝线相比，单丝具有轻度的 eNOS 活性。糖尿病组的 iNOS 活性高于对照组，单丝、丝线和肠线缝线的活性在第 7 天为轻度、重度和中度。在第 14 天，糖尿病单丝组无 iNOS 活性。
所有材料都具有良好的耐受性潜力，但单丝似乎对糖尿病患者的伤口愈合具有更有益的影响。
