Urologic Oncology Clinic, Center for Specific Organs Cancer, National Cancer Center, Goyang, Republic of Korea.
J Surg Oncol. 2010 Feb 1;101(2):145-8. doi: 10.1002/jso.21445.
To determine whether the presence of prostate stem cell antigen (PSCA) mRNA in peripheral blood can predict biochemical recurrence (BCR) after radical prostatectomy in patients with high-risk prostate cancer.
High-risk disease was defined based on the presence of any one of the following three risk factors: prostate-specific antigen (PSA) > or = 20 ng/ml, a biopsy Gleason score (GS) > or = 8, or clinical stage > or = T2c. Nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect PSCA mRNA-bearing cells in peripheral blood. The relationship between PSCA detection and BCR after surgery was evaluated.
Of the 250 patients, 103 (36.8%) with high-risk disease were included in the analysis. PSCA-mRNA was detected in 17 (16.5%) of these patients. Patients with high GS (> or = 7) tended to be PSCA-mRNA positive status (P = 0.045). Over a median 23 months of follow-up (range 3-47 months), BCR developed in 27 patients (26.2%). Cox regression hazards model analysis revealed that a RT-PCR PSCA positivity (HR, 4.549; 95%CI, 1.685-12.279; P = 0.003) independently increased the risk of BCR.
The presence of PSCA mRNA in peripheral blood is a significant predictor of BCR after radical prostatectomy in high-risk prostate cancer.
确定外周血前列腺干细胞抗原（PSCA）mRNA 的存在是否能预测高危前列腺癌患者根治性前列腺切除术后的生化复发（BCR）。
高危疾病的定义基于以下三个危险因素中的任何一个：前列腺特异性抗原（PSA）≥20ng/ml，活检 Gleason 评分（GS）≥8，或临床分期≥T2c。采用巢式逆转录聚合酶链反应（RT-PCR）检测外周血中携带 PSCA mRNA 的细胞。评估 PSCA 检测与术后 BCR 的关系。
在 250 例患者中，有 103 例（36.8%）患有高危疾病，包括在分析中。这些患者中有 17 例（16.5%）检测到 PSCA-mRNA。高 GS（≥7）的患者倾向于 PSCA-mRNA 阳性状态（P=0.045）。在中位 23 个月的随访期间（范围 3-47 个月），27 例患者（26.2%）发生 BCR。Cox 回归风险模型分析显示，RT-PCR PSCA 阳性（HR，4.549；95%CI，1.685-12.279；P=0.003）独立增加了 BCR 的风险。
外周血中 PSCA mRNA 的存在是高危前列腺癌根治性前列腺切除术后 BCR 的一个显著预测因子。
