Pigment Clinic, National Skin Centre, Singapore.
Dermatol Surg. 2010 Feb;36(2):203-7. doi: 10.1111/j.1524-4725.2009.01423.x. Epub 2009 Dec 21.
Vitiligo is a disfiguring depigmenting dermatosis that affects approximately 0.5% to 1% of the general population regardless of race and sex. In patients with stable vitiligo who fail conventional therapies, surgical transplant offers a viable alternative. Noncultured cellular grafting offers the advantage of repigmenting vitiligo 5 to 10 times the size of the donor skin and can be completed on the same day on an outpatient basis. In recent years, ways to simplify this procedure have been explored, including the use of commercially available kits.
To simplify the extraction of epidermal cells from donor skin using a 6-well plate and to evaluate the clinical efficacy of this simplified technique in repigmenting stable vitiligo and piebaldism.
Four patients with focal or segmental vitiligo and one with piebaldism were treated using the simplified noncultured cellular grafting protocol. Percentage of repigmentation 6 months after grafting was objectively measured using digital contour mapping.
Patients with stable segmental or focal vitiligo achieved 65% to 92% repigmentation 6 months after grafting; the treated sites involved face or limbs. The patient with piebaldism achieved 86% repigmentation. One year after grafting, the extent of repigmentation remained for all patients.
This set-up is simple and inexpensive; it reduces cell preparation time, amount of reagents used, and costs, and obviates the need of a laboratory for extraction of epidermal cells.
白癜风是一种影响约 0.5%至 1%的普通人群的毁容性脱色素皮肤病，无论种族和性别如何。对于常规治疗失败的稳定型白癜风患者，手术移植提供了一种可行的替代方法。非培养细胞移植具有使白癜风复色 5 至 10 倍于供皮区的优势，并且可以在门诊的同一天完成。近年来，人们一直在探索简化该程序的方法，包括使用商业上可获得的试剂盒。
使用 6 孔板简化从供皮区提取表皮细胞的方法，并评估该简化技术在复色稳定型白癜风和斑驳病中的临床疗效。
采用简化的非培养细胞移植方案治疗 4 例局限性或节段性白癜风患者和 1 例斑驳病患者。移植后 6 个月，使用数字轮廓图客观测量复色率。
稳定的节段性或局限性白癜风患者在移植后 6 个月实现了 65%至 92%的复色；治疗部位涉及面部或四肢。斑驳病患者实现了 86%的复色。移植后 1 年，所有患者的复色范围均保持不变。
这种方案简单且经济实惠；它减少了细胞准备时间、试剂用量和成本，并且无需实验室提取表皮细胞。
