OraSure Technologies, Research and Development, Bethlehem, Pennsylvania 18015, USA.
J Anal Toxicol. 2009 Nov-Dec;33(9):569-77. doi: 10.1093/jat/33.9.569.
Analytical methods for measuring multiple licit and illicit drugs and metabolites in oral fluid require high sensitivity, specificity, and accuracy. With the limited volume available for testing, comprehensive methodology is needed for simultaneous measurement of multiple analytes in a single aliquot. This report describes the validation of a semi-automated method for the simultaneous extraction, identification, and quantitation of 21 analytes in a single oral fluid aliquot. The target compounds included are amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxy-amphetamine, 3,4-methylenedioxyethylamphetamine, pseudoephedrine, cocaine, benzoylecgonine, codeine, norcodeine, 6-acetylcodeine, morphine, 6-acetylmorphine, hydrocodone, norhydrocodone, dihydrocodeine, hydromorphone, oxycodone, noroxycodone, oxymorphone, and phencyclidine. Oral fluid specimens were collected with the Intercept device and extracted by solid-phase extraction (SPE). Drug recovery from the Intercept device averaged 84.3%, and SPE extraction efficiency averaged 91.2% for the 21 analytes. Drug analysis was performed by liquid chromatography-tandem mass spectrometry in the positive electrospray mode using ratios of qualifying product ions within +/-25% of calibration standards. Matrix ion suppression ranged from -57 to 8%. The limit of quantitation ranged from 0.4 to 5 ng/mL using 0.2 mL of diluted oral fluid sample. Application of the method was demonstrated by testing oral fluid specimens from drug abuse treatment patients. Thirty-nine patients tested positive for various combinations of licit and illicit drugs and metabolites. In conclusion, this validated method is suitable for simultaneous measurement of 21 licit and illicit drugs and metabolites in oral fluid.
用于测量唾液中多种合法和非法药物及其代谢物的分析方法需要具有高灵敏度、特异性和准确性。由于可供测试的样本量有限，因此需要综合方法来同时测量单个样本中的多种分析物。本报告介绍了一种半自动化方法的验证，该方法可用于同时提取、鉴定和定量单个唾液样本中的 21 种分析物。目标化合物包括苯丙胺、甲基苯丙胺、3,4-亚甲基二氧基苯丙胺、3,4-亚甲基二氧基苯丙胺、3,4-亚甲基二氧基乙基苯丙胺、伪麻黄碱、可卡因、苯甲酰古柯碱、可待因、去甲可待因、6-乙酰可待因、吗啡、6-乙酰吗啡、氢可酮、去氢可酮、二氢可待因、氢吗啡酮、羟考酮、去甲羟考酮、羟吗啡酮和苯环己哌啶。唾液样本用 Intercept 装置收集，并用固相萃取 (SPE) 提取。从 Intercept 装置中回收的药物平均为 84.3%，21 种分析物的 SPE 提取效率平均为 91.2%。药物分析通过正电喷雾模式下的液相色谱-串联质谱法进行，使用校准标准品正负 25%范围内的定性产物离子比值。基质离子抑制范围为-57 至 8%。使用 0.2 毫升稀释唾液样本，定量下限范围为 0.4 至 5 ng/mL。通过对药物滥用治疗患者的唾液样本进行测试，证明了该方法的适用性。39 名患者对各种合法和非法药物及其代谢物呈阳性反应。总之，该验证方法适用于同时测量唾液中的 21 种合法和非法药物及其代谢物。
