Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Republic of Korea.
Biochem Biophys Res Commun. 2010 Jan 22;391(4):1687-92. doi: 10.1016/j.bbrc.2009.12.131. Epub 2009 Dec 27.
A novel alpha-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. alpha-iso-cubebenolinhibited LPS-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production. Consistent with these findings, alpha-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. alpha-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-kappaB p65 subunit. Furthermore, alpha-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel alpha-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin.
从五味子果实中分离得到了具有抗炎作用的新型 alpha-iso-cubebenol，可抑制脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞产生一氧化氮（NO）和前列腺素 E2（PGE2）。与这些发现一致，alpha-iso-cubebenol 还可浓度依赖性地降低 LPS 诱导的诱导型一氧化氮合酶和环氧化酶-2 的蛋白和 mRNA 表达。alpha-iso-cubebenol 还抑制 LPS 诱导的 NF-kappaB p65 亚基核易位。此外，alpha-iso-cubebenol 抑制 LPS 诱导的 ERK、JNK 和 p38 激酶的磷酸化。由于新型 alpha-iso-cubebenol 可阻断 LPS 诱导的巨噬细胞中几种促炎介质的产生，因此该分子可用于开发针对细菌感染或内毒素的抗炎药物。
