Chinese PLA General Hospital, Department of Orthopaedics, Beijing 100853, China.
Acta Biomater. 2010 Jun;6(6):2189-99. doi: 10.1016/j.actbio.2009.12.042. Epub 2009 Dec 28.
The objective of this study was to investigate the effects of spatial structure and crystalline phase on the biological performance of collagen-hydroxyapatite (Col-HA) composite prepared by biomineralization crystallization. Two types of Col-HA composites were prepared using mineralization crystallization (MC composites) and pre-crystallization (PC composites), respectively. Structural characteristics were analyzed by scanning electron microscopy and transmission electron microscopy. Surface elemental compositions were measured by electron spectroscopy for chemical analysis (ESCA). These composites were used in in vivo repair of bone defects. The effects of the crystalline phase on the biological performance of Col-HA composites were investigated using radionuclide bone scan, histopathology and morphological observation. It was observed that in MC composites, HA was located on the surface of the collagen fibers and aggregated into crystal balls, whereas HA in PC composites was scattered among the collagen fibers. ESCA showed that phosphorus and calcium were 8.99% and 17.56% on MC composite surface, compared with 4.39% and 5.86% on the PC composite surface. In vivo bone defect repair experiments revealed that radionuclide uptake was significantly higher in the area implanted with the PC composite than in the contralateral area implanted with the MC composite. Throughout the whole repair process, the PC composite proved to be superior to the MC composite with regard to capillary-forming capacity and the amount of newly formed bone tissue. So it could be concluded that HA placement on collagen fibers affected the biological performance of Col-HA composites. Pre-crystallization made HA scattered among collagen fibers, creating a better structure for bone defect repair in comparison with MC Col-HA composites.
本研究旨在探讨空间结构和晶相对通过生物矿化结晶制备的胶原-羟基磷灰石（Col-HA）复合材料生物学性能的影响。分别通过矿化结晶（MC 复合材料）和预结晶（PC 复合材料）制备了两种 Col-HA 复合材料。通过扫描电子显微镜和透射电子显微镜分析结构特征。通过电子能谱分析（ESCA）测量表面元素组成。将这些复合材料用于体内骨缺损修复。通过放射性核素骨扫描、组织病理学和形态观察研究了晶相对 Col-HA 复合材料生物学性能的影响。结果表明，在 MC 复合材料中，HA 位于胶原纤维表面并聚集形成晶球，而 PC 复合材料中的 HA 则分散在胶原纤维之间。ESCA 显示 MC 复合材料表面的磷和钙分别为 8.99%和 17.56%，而 PC 复合材料表面的磷和钙分别为 4.39%和 5.86%。体内骨缺损修复实验表明，植入 PC 复合材料的区域放射性核素摄取明显高于植入 MC 复合材料的对侧区域。在整个修复过程中，PC 复合材料在成血管能力和新形成的骨组织量方面均优于 MC 复合材料。因此，可以得出结论，HA 在胶原纤维上的位置影响 Col-HA 复合材料的生物学性能。与 MC Col-HA 复合材料相比，预结晶使 HA 分散在胶原纤维之间，为骨缺损修复创造了更好的结构。
