一种由单克隆抗体（PXM1a/207B）检测到的新型57 kDa过氧化物酶体膜多肽。

A novel 57 kDa peroxisomal membrane polypeptide detected by monoclonal antibody (PXM1a/207B).

作者信息

Imanaka T, Lazarow P B, Takano T

机构信息

Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.

出版信息

Biochim Biophys Acta. 1991 Feb 25;1062(2):264-70. doi: 10.1016/0005-2736(91)90401-s.

DOI:10.1016/0005-2736(91)90401-s

PMID:2004113

Abstract

BALB/c mice were immunized with peroxisomal membranes prepared from rat liver. Spleen cells were fused with myeloma cells (P3/U1) and the hybridomas were selected using peroxisomal membranes. A monoclonal antibody (PXM1a/207B) which recognized peroxisomal membranes was selected. Using the antibody, a novel 57 kDa polypeptide was identified in the peroxisomal membrane fraction. Immunoblot analysis of the subcellular fractions demonstrated that the 57 kDa polypeptide was present exclusively in peroxisomal membranes. The 57 kDa polypeptide was partially digested by trypsin and chymotrypsin under conditions where peroxisomal particles remained intact, indicating that the polypeptide is exposed to the cytosolic face of the peroxisomal membrane. The amount of 57 kDa polypeptide increased in parallel with proliferation of peroxisomes by administration of clofibrate.

摘要

用从大鼠肝脏制备的过氧化物酶体膜免疫BALB/c小鼠。脾细胞与骨髓瘤细胞（P3/U1）融合，并用过氧化物酶体膜筛选杂交瘤。筛选出一种识别过氧化物酶体膜的单克隆抗体（PXM1a/207B）。使用该抗体，在过氧化物酶体膜组分中鉴定出一种新的57 kDa多肽。亚细胞组分的免疫印迹分析表明，57 kDa多肽仅存在于过氧化物酶体膜中。在过氧化物酶体颗粒保持完整的条件下，57 kDa多肽被胰蛋白酶和胰凝乳蛋白酶部分消化，表明该多肽暴露于过氧化物酶体膜的胞质面。通过给予氯贝丁酯，57 kDa多肽的量与过氧化物酶体的增殖平行增加。