Jiangsu Key Laboratory of Molecular Medicine & The Center for Reproductive medicine, Drum Tower Hospital Affiliated to Medical School, Nanjing University, Nanjing, 210093, China.
J Assist Reprod Genet. 2010 Jan;27(1):23-8. doi: 10.1007/s10815-009-9377-8. Epub 2009 Dec 30.
This study was to evaluate whether polymorphisms of TCF7L2 (rs7903146) and HHEX (rs1111875) genes responsible for insulin secretion are associated with the polycystic ovary syndrome (PCOS) in Chinese people.
326 PCOS patients and 290 healthy individuals as controls were studied. Blood samples were obtained for DNA analyses and hormone measurements. Genotyping of the TCF7L2 (rs7903146) and HHEX (rs1111875) genes was carried out by the polymerase chain reaction-restriction fragment length polymorphism method.
We did not find statistically significant differences in the distribution of the TCF7L2 rs7903146 and HHEX rs1111875 polymorphisms between the Chinese women with PCOS and the controls. Levels of hormones such as insulin, FSH, LH, LH/FSH, P, T and E2 were also similar between the different genotypes of the genes TCF7L2 and HHEX, respectively, which was confirmed within either the PCOS subjects or controls.
There was no association of either of the two variants, rs7903146 of TCF7L2 and rs1111875 of HHEX, with the occurrence of PCOS in the Chinese population.
本研究旨在评估负责胰岛素分泌的 TCF7L2（rs7903146）和 HHEX（rs1111875）基因多态性是否与中国人的多囊卵巢综合征（PCOS）有关。
研究纳入 326 例 PCOS 患者和 290 名健康对照者。采集血样进行 DNA 分析和激素测量。采用聚合酶链反应-限制性片段长度多态性方法检测 TCF7L2（rs7903146）和 HHEX（rs1111875）基因的多态性。
我们未发现 PCOS 患者与对照组之间 TCF7L2 rs7903146 和 HHEX rs1111875 多态性的分布存在统计学差异。TCF7L2 和 HHEX 基因不同基因型之间的激素水平（如胰岛素、FSH、LH、LH/FSH、P、T 和 E2）也相似，这在 PCOS 患者或对照组中均得到了证实。
在中国人群中，TCF7L2 的 rs7903146 或 HHEX 的 rs1111875 这两种变异与 PCOS 的发生均无关联。
