电针对 DNP-KLH 免疫小鼠的全基因组表达谱。
The genome-wide expression profile of electroacupuncture in DNP-KLH immunized mice.
机构信息
Brain Korea 21 Project for Medical Science, Yonsei University, Seoul, 130-701, Republic of Korea.
出版信息
Cell Mol Neurobiol. 2010 May;30(4):631-40. doi: 10.1007/s10571-009-9487-y. Epub 2009 Dec 30.
Abstract
Previously, we demonstrated that electoracupuncture (EA) suppressed allergic reactions in DNP-KLH immunized mice. In this study, the mechanisms by which EA induces immunomodulation in the immunized mice were evaluated by genome-wide microarray analysis. The anti-allergic effects of EA in DNP-KLH immunized mice were confirmed by analyzing antigen specific IgE using ELISA. Microarray analysis, followed by real time RT-PCR validation, revealed that Th1 and Th17 cytokine-, opioid peptide-, and anti-apoptosis-related genes were up-regulated upon treatment with EA. In addition, significant decreases in Th2 cytokine-, MAPK signaling pathway-, and apoptosis-related genes were observed following EA treatment.
摘要
此前，我们已经证明电针对 DNP-KLH 免疫的小鼠的过敏反应具有抑制作用。在本研究中，通过全基因组微阵列分析评估了电针对免疫小鼠产生免疫调节的机制。通过 ELISA 分析抗原特异性 IgE 来确认电针对 DNP-KLH 免疫的小鼠的抗过敏作用。微阵列分析，随后进行实时 RT-PCR 验证，表明电针治疗后 Th1 和 Th17 细胞因子、阿片肽和抗细胞凋亡相关基因上调。此外，电针治疗后观察到 Th2 细胞因子、MAPK 信号通路和凋亡相关基因显著下调。
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