Nestle Research Center, Nestec Ltd PO Box 44, CH-1000 Lausanne 26, Switzerland.
J Agric Food Chem. 2010 Jan 27;58(2):729-33. doi: 10.1021/jf903032q.
A method for the specific detection and quantification of recombinant bovine somatotropin (rbST) in bovine blood has been validated according to criteria described in the EU Commission Decision 2002/657/EC. The method is based on a thorough purification procedure followed with the detection by LC-ESI-MS/MS of the tryptic N-terminal peptide specific of the rbST. The recombinant equine somatotropin (reST) is used as internal standard. Performance of the method was assessed based on specificity, linearity, trueness and repeatability. Decision limit (CCalpha) and detection capability (CCbeta) were found to be 2.5 ng mL(-1) and 6.8 ng mL(-1), respectively. This method was subsequently applied to the analysis of serum and plasma collected from two different animals treated with 500 mg of rbST. No significant variations were observed when analyzing either serum or plasma, but an important difference between animals was encountered. In all cases, recombinant bovine somatotropin was still detected two weeks after administration.
已经根据欧盟委员会第 2002/657/EC 号决定中描述的标准，验证了一种用于在牛血液中特异性检测和定量重组牛生长激素（rbST）的方法。该方法基于彻底的纯化程序，然后通过 LC-ESI-MS/MS 检测 rbST 的特异性胰蛋白酶 N 端肽。重组马生长激素（reST）用作内标。该方法的性能基于特异性、线性、准确性和重复性进行评估。发现决策限（CCalpha）和检测能力（CCbeta）分别为 2.5 ng/mL 和 6.8 ng/mL。随后将该方法应用于分析用 500 mg rbST 处理的两种不同动物的血清和血浆。分析血清或血浆时均未观察到显著差异，但在动物之间存在重要差异。在所有情况下，给药两周后仍能检测到重组牛生长激素。
