Functional Genomics Technology Team, Omics Science Center (OSC), RIKEN Yokohama Institute, Kanagawa, Japan.
Biotechniques. 2009 Dec;47(6):1041-3. doi: 10.2144/000113249.
We developed a method for selective purification of DNA using the cationic detergent, cetyltrimethylammonium bromide (CTAB), accompanied with urea and controlled high-salt (NaCl) concentration. This method is effective for rapid separation of DNA fragments from artifacts such as PCR primer dimers or ligation adapters. The CTAB-associated purification completely removed the short PCR artifacts and primers, as well as enzymes and buffer, while recovering a sufficient quantity of amplicons for subsequent experiments such as preparation of libraries. This method could also be applied to the fractionation of nucleic acids generated by other types of reactions.
我们开发了一种使用阳离子去污剂十六烷基三甲基溴化铵 (CTAB) 与尿素和控制高盐 (NaCl) 浓度相结合的方法，用于选择性地纯化 DNA。该方法可有效分离 PCR 引物二聚体或连接接头等人工产物中的 DNA 片段。CTAB 相关的纯化方法可完全去除短 PCR 人工产物和引物以及酶和缓冲液，同时回收足够量的扩增子，用于后续实验，如文库制备。该方法还可应用于其他类型反应产生的核酸的分离。
