Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany.
Anal Biochem. 2011 Mar 1;410(1):155-7. doi: 10.1016/j.ab.2010.11.029. Epub 2010 Nov 25.
Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications.
聚合酶链反应（PCR）的分区化减少了伪影，特别是在扩增复杂文库时。它允许以无偏倚的方式对复杂混合物中的模板进行克隆扩增。在这里，我们描述了一种在油包水乳剂（ePCR）中进行 PCR 的快速、直接和简单的方案，包括通过 DNA 纯化进行样品回收。此外，不需要特殊的实验室设备，并且使用了廉价的组件。因此，我们灵活的方案允许 ePCR 易于在广泛的应用中在日常常规实验中实施。
