Kang Hye-Min, Jin Jizi, Lee Seungjoo, Ryu Jonghoon, Park Chan
Department of Anatomy and Neurobiology, Biomedical Science Institute, School of Medicine, Seoul 130-701, Korea.
Neuroreport. 2010 Feb 17;21(3):179-84. doi: 10.1097/WNR.0b013e328334884c.
We present the photochemically induced olfactory bulbectomy (P-bulbectomy) as a novel method to ablate the olfactory bulb thus inducing an animal model of depression. The photosensitizer Rose Bengal was injected through the tail vein and then the cool halogen light illuminated the skull region overlying of the olfactory bulb for 10 min. Two weeks after surgery, P-bulbectomy had completely removed olfactory bulb uniformly in all animals. P-bulbectomy induced typical depression-related behaviors such as hyperactivity in the open field test and an enhancement of immobility time and in the forced swimming test. Depression-related neurohistological phenomenon was also seen; reduction of choline-acetyltransferase-positive cell numbers in the medial septum and a decline in cell proliferation in the dentate gyrus of hippocampus. This study shows that P-bulbectomy may be a convenient and reproducible experimental method to produce an animal model of depression.
我们提出光化学诱导嗅球切除术（P-嗅球切除术）作为一种消融嗅球从而诱导抑郁症动物模型的新方法。将光敏剂孟加拉玫瑰红通过尾静脉注射，然后用冷卤素灯照射嗅球上方的颅骨区域10分钟。手术后两周，P-嗅球切除术在所有动物中均完全均匀地切除了嗅球。P-嗅球切除术诱导了典型的抑郁相关行为，如旷场试验中的多动以及强迫游泳试验中不动时间的增加。还观察到了与抑郁相关的神经组织学现象；内侧隔区胆碱乙酰转移酶阳性细胞数量减少以及海马齿状回细胞增殖下降。本研究表明，P-嗅球切除术可能是一种方便且可重复的实验方法，用于建立抑郁症动物模型。
