Department of Pathology (R-71), Miller School of Medicine, University of Miami, Miami, Florida 33101, USA.
Cytometry B Clin Cytom. 2010 May;78(3):176-82. doi: 10.1002/cyto.b.20509.
Enhanced expression of aldehyde dehydrogenase 1 (ALDH1) and phenotypic markers (CD44(+)/CD24(-)) in stem cells from breast tumors has been reported. This study was undertaken to monitor expression of these markers in cells from body cavity fluids of female patients suspected to have a malignancy.
Cells from peritoneal and pleural fluids of 100 female patients were examined by diagnostic cytology and analyzed by laser flow cytometry for enhanced ALDH1 expression. Cells from 36 body cavity fluids with ALDH1(bright) fluorescence were then analyzed for the expression of CD44 and CD24 markers.
In samples positive for malignancy, ALDH1(bright) cells with both SSC(low) and SSC(high) were seen. In 15 body cavity fluids positive for malignancy, the percentage of ALDH1(bright) cells ranged from 0.26 to 6.34% of the total cells. The percentage of ALDH1(bright) cells with CD44(+)/CD24(-) expression in these samples ranged from 0.02 to 3.66%. ALDH1(bright) cells with CD44(+)/CD24(-) expression were also present in body cavity fluids of patients in whom diagnostic cytology could not detect any malignancy. However, the percentage of ALDH1(bright) and CD44(+)/CD24(-) cells amongst the 21 body cavity fluids with negative cytology was lower than that of samples with malignancy.
Expression of ALDH1(bright) and the CD44(+)/CD24(-) phenotype in body cavity fluids in which diagnostic cytology could not find any malignant cells suggests that this phenotype may not be restricted to the putative breast tumor stem cells. It is possible that only subsets of cells with this phenotype are the putative breast tumor stem cells.
已有报道称，肿瘤干细胞中醛脱氢酶 1（ALDH1）和表型标志物（CD44(+)/CD24(-)）的表达增强。本研究旨在监测疑似恶性肿瘤女性患者体腔液中这些标志物的表达。
对 100 例女性患者的腹腔和胸腔液中的细胞进行诊断细胞学检查，并通过激光流式细胞术分析增强的 ALDH1 表达。然后对 36 个体腔液中具有 ALDH1（明亮）荧光的细胞进行 CD44 和 CD24 标志物的表达分析。
在恶性肿瘤阳性样本中，观察到具有低 SSC 和高 SSC 的 ALDH1(bright)细胞。在 15 例恶性肿瘤阳性的体腔液样本中，ALDH1(bright)细胞的百分比范围为总细胞的 0.26%至 6.34%。这些样本中具有 CD44(+)/CD24(-)表达的 ALDH1(bright)细胞的百分比范围为 0.02%至 3.66%。在诊断细胞学无法检测到任何恶性肿瘤的患者的体腔液中也存在具有 CD44(+)/CD24(-)表达的 ALDH1(bright)细胞。然而，在 21 例细胞学阴性的体腔液中，ALDH1(bright)和 CD44(+)/CD24(-)细胞的百分比低于有恶性肿瘤的样本。
在诊断细胞学无法发现任何恶性细胞的体腔液中表达 ALDH1(bright)和 CD44(+)/CD24(-)表型表明，这种表型可能不仅限于假定的乳腺癌干细胞。具有这种表型的细胞亚群可能才是假定的乳腺癌干细胞。
