Department of Biochemistry, School of Medicine, University of Crete, Heraklion, Greece.
Cancer Lett. 2010 Feb 1;288(1):99-106. doi: 10.1016/j.canlet.2009.06.027. Epub 2009 Jul 19.
The CD44(+)/CD24(-/low) and ALDH1(+) cell phenotypes are associated with stemness and enhanced tumorigenic potential in breast cancer. We assessed the expression of CD44, CD24 and ALDH1 on tumor cells circulating in the peripheral blood (CTCs) of patients with metastatic breast cancer using triple-marker immunofluorescence microscopy. Among a total of 1439 CTCs identified in 20 (66.7%) out of 30 patients, 35.2% had the stem-like/tumorigenic phenotype CD44(+)/CD24(-/low), whereas 17.7% of the CTCs analyzed in seven patients, were ALDH1(high)/CD24(-/low). In conclusion, we report the existence of a subpopulation of CTCs with putative stem cell progenitor phenotypes in patients with metastatic breast cancer.
CD44(+) / CD24(-/low) 和 ALDH1(+) 细胞表型与乳腺癌的干性和增强的肿瘤发生潜能相关。我们使用三标记免疫荧光显微镜评估了转移性乳腺癌患者外周血循环肿瘤细胞 (CTC) 中 CD44、CD24 和 ALDH1 的表达。在 30 名患者中的 20 名（66.7%）共鉴定出 1439 个 CTC 中，有 35.2% 的 CTC 具有干性/肿瘤发生表型 CD44(+) / CD24(-/low)，而在 7 名患者中分析的 17.7% 的 CTC 为 ALDH1(high) / CD24(-/low)。总之，我们报告了转移性乳腺癌患者 CTC 中存在具有潜在干细胞祖细胞表型的亚群。
