组蛋白 H3 在肠道 Caco-2 细胞中 SLC2A5 基因上赖氨酸 4 的甲基化参与 SLC2A5 的表达。
Histone H3 methylation at lysine 4 on the SLC2A5 gene in intestinal Caco-2 cells is involved in SLC2A5 expression.
机构信息
Laboratory of Nutritional Physiology, The University of Shizuoka, Graduate School of Nutritional and Environmental Sciences and Global COE, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan.
出版信息
Biochem Biophys Res Commun. 2010 Jan 29;392(1):16-21. doi: 10.1016/j.bbrc.2009.12.136. Epub 2009 Dec 31.
Histone H3 methylation at lysine 4 (K4) is associated with euchromatic regions and is thought to be important for the transcriptional activation of genes during differentiation. In this study, we found that di- and tri-methylation of histone H3 at K4 and acetylation of histones H3 and H4 from the promoter/enhancer to the transcribed region close to the transcription initiation site of the solute carrier family 2, member 5 (SLC2A5) gene, and its expression, were induced by differentiation of intestine-like Caco-2 cells. These effects were accompanied by contact inhibition of cell growth of these cells. Furthermore, these modifications were induced by co-treatment with a synthetic glucocorticoid hormone dexamethasone and a p44/42 mitogen-activated protein kinase inhibitor PD89059. Our results suggest that methylation of histone H3 at K4 and acetylation of histones H3 and H4 are involved in SLC2A5 gene induction associated with intestinal differentiation of Caco-2 cells.
组蛋白 H3 在赖氨酸 4 位的甲基化（K4）与常染色质区域相关，被认为在分化过程中基因的转录激活中很重要。在这项研究中，我们发现二甲基化和三甲基化的组蛋白 H3 在 K4 处，以及从启动子/增强子到靠近溶质载体家族 2 成员 5（SLC2A5）基因转录起始位点的转录区域的组蛋白 H3 和 H4 的乙酰化，以及 SLC2A5 基因的表达，在肠样 Caco-2 细胞的分化过程中被诱导。这些效应伴随着这些细胞生长的接触抑制。此外，这些修饰可通过用合成的糖皮质激素激素地塞米松和 p44/42 丝裂原活化蛋白激酶抑制剂 PD89059 共同处理来诱导。我们的结果表明，组蛋白 H3 在 K4 位的甲基化和组蛋白 H3 和 H4 的乙酰化参与了与 Caco-2 细胞肠分化相关的 SLC2A5 基因的诱导。
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