Agence Française de sécurité sanitaire des produits de santé, 93285 Saint-Denis Cedex, France.
J Virol Methods. 2010 Apr;165(1):105-7. doi: 10.1016/j.jviromet.2009.12.007. Epub 2009 Dec 28.
We evaluated the sensitivity for the anti-HIV antibody detection of the 8 rapid disposable tests (RDTs) EC approved in 2008.
A panel of 100 native serum samples collected from HIV-1 subtype B or non-B, HIV-1 group O or HIV-2-infected patient and 3 commercial seroconversion panels were tested. 50 sera from HIV-negative patients were included to blind reading and RDT specificity.
All native samples HIV-1 subtype B were reactive; an HIV-1 non-B subtype gave false-negative results with 3 of the 8 tests. False-negative results on HIV-1 group O samples were observed with one RDT. All the HIV-2 samples were detected. Seroconversion sample reactivity ranged from 60 to 86.7% according to the tests.
Despite their lower performances relative to ELISA tests during the HIV seroconversion period, RDT may be of interest in case of chronic infection.
我们评估了在 2008 年被 EC 批准的 8 种快速一次性检测（RDT）对 HIV 抗体检测的敏感性。
用从 HIV-1 亚型 B 或非 B、HIV-1 组 O 或 HIV-2 感染患者中收集的 100 个原始血清样本和 3 个商业血清转换面板进行了检测。纳入 50 份来自 HIV 阴性患者的血清进行盲法阅读和 RDT 特异性检测。
所有的 HIV-1 亚型 B 原始样本均呈阳性反应；8 种检测中有 3 种对 HIV-1 非 B 亚型的检测结果为假阴性。有一种 RDT 对 HIV-1 组 O 样本的检测结果为假阴性。所有 HIV-2 样本均被检出。根据不同的检测方法，血清转换样本的反应性范围为 60%至 86.7%。
尽管在 HIV 血清转换期间，RDT 的性能相对 ELISA 检测较低，但在慢性感染的情况下，RDT 可能具有一定的参考价值。
