葫芦脲与血根碱包结作用的光谱荧光法研究及其分析应用。
Study on the inclusion interaction of cucurbit[n]urils with sanguinarine by spectrofluorimetry and its analytical application.
机构信息
Analytical and Testing Center, Shanxi Normal University, Shanxi, Linfen 041004, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Feb;75(2):912-7. doi: 10.1016/j.saa.2009.12.036. Epub 2009 Dec 14.
The characteristics of host-guest complexation between cucurbit[n]uril (CB[n], n=5, 6, 7, 8) and sanguinarine (SA) were investigated by spectrofluorimetry. The result showed that CB[n] (n=5, 6, 7, 8) reacted with SA to form an inclusion complex. At the optimum reaction conditions, the rectilinear calibration graphs were obtained in the range from 0.1 to 2800 ng mL(-1) for the investigated drug, with correlation coefficients 0.9998, 0.9997, 0.9999 and 0.9997 and detection limits 0.052, 0.26, 0.03 and 0.058 ng mL(-1) using CB[5], CB[6], CB[7], and CB[8], respectively. The apparent association constants of the complexes were 1.23 x 10(4), 6.73 x 10(3), 4.53 x 10(4) and 1.21 x 10(4) L mol(-1), for the reagents in the same order. The result demonstrated that CB[7] exhibited a higher fluorescence signal than CB[5], CB[6], and CB[8]. The proposed method was successfully applied for the determination of the drug in human urine and serum samples.
通过荧光光谱法研究了葫芦[n]脲（CB[n]，n=5、6、7、8）与血根碱（SA）之间的主客体络合特性。结果表明，CB[n]（n=5、6、7、8）与 SA 反应形成包合物。在最佳反应条件下，在所研究药物的 0.1 至 2800ng mL(-1)范围内，得到了线性校准曲线，相关系数分别为 0.9998、0.9997、0.9999 和 0.9997，检测限分别为 0.052、0.26、0.03 和 0.058ng mL(-1)，使用 CB[5]、CB[6]、CB[7]和 CB[8]。复合物的表观缔合常数分别为 1.23 x 10(4)、6.73 x 10(3)、4.53 x 10(4)和 1.21 x 10(4)L mol(-1)，试剂按相同顺序排列。结果表明，CB[7]比 CB[5]、CB[6]和 CB[8]表现出更高的荧光信号。该方法成功应用于人尿和血清样品中药物的测定。
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