Analytical and Testing Center, Shanxi Normal University, Shanxi Linfen 041004, PR China.
Talanta. 2010 Mar 15;80(5):1939-44. doi: 10.1016/j.talanta.2009.10.049. Epub 2009 Oct 29.
The supramolecular interaction of a homologous series of cucurbit[n]uril (CB[n], n=5, 6, 7, 8) hosts and coptisine (COP) was studied by spectrofluorimetry. All of the CB[n]s were found to react with COP to form 1:1 host-guest stable complexes and the fluorescence intensity of the complexes was greatly enhanced. The apparent association constants of the complexes were 1.44 x 10(4), 1.28 x 10(4), 1.86 x 10(4) and 1.26 x 10(4)L mol(-1) for CB[5], CB[6], CB[7] and CB[8], respectively. In addition, CB[5] and CB[7] exhibited a higher fluorescence signal than CB[6] and CB[8]. The fluorescence intensity of the complex with CB[7] was enhanced 70-fold compared to that of the studied drug itself. Based on the significant enhancement of fluorescence intensity of supramolecular complex, a simple, rapid, highly sensitive, and selective spectrofluorimetric method was developed for the determination of COP in aqueous solution in the presence of CB[7]. At the optimum reaction conditions, a linear relationship was obtained in the range from 0.05 to 1700 ng mL(-1) with a detection limit of 0.012 ng mL(-1). The proposed method was successfully applied for the determination of the drug in urine and serum samples.
通过光谱荧光法研究了一系列同系物的葫芦脲（CB[n]，n=5、6、7、8）主体与小檗碱（COP）的超分子相互作用。所有 CB[n]都被发现与 COP 反应形成 1:1 的主客体稳定配合物，并且配合物的荧光强度大大增强。配合物的表观结合常数分别为 1.44×10^4、1.28×10^4、1.86×10^4 和 1.26×10^4 L mol^-1，对于 CB[5]、CB[6]、CB[7]和 CB[8]。此外，CB[5]和 CB[7]表现出比 CB[6]和 CB[8]更高的荧光信号。与研究药物本身相比，与 CB[7]形成的配合物的荧光强度增强了 70 倍。基于超分子配合物荧光强度的显著增强，建立了一种在 CB[7]存在下测定水溶液中 COP 的简单、快速、高灵敏、选择性光谱荧光法。在最佳反应条件下，在 0.05 至 1700 ng mL^-1 范围内得到线性关系，检测限为 0.012 ng mL^-1。该方法成功应用于尿液和血清样品中药物的测定。
