Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing, China.
Arch Environ Contam Toxicol. 2010 Jul;59(1):129-36. doi: 10.1007/s00244-009-9456-y. Epub 2010 Jan 1.
In the present study, the thermotaxis model was used to evaluate the effects of metal exposure at different concentrations on associative learning behavior in nematodes. The examined nematodes were cultured at 25 or 17 degrees C, and then shifted to 20 degrees C condition. Based on the ability of nematodes to trace the temperature of 20 degrees C, exposure to 10 microM of all examined metals and 2.5 microM Pb and Hg caused significant decrease of associative learning behavior at time intervals of 5 and 18 h; however, exposure to 2.5 microM Cu, Zn, and Ag did not influence associative learning behavior. Moreover, exposure to 2.5 and 10 microM of examined metals did not influence body bend and thermotaxis to cultivation temperature, whereas exposure to 50 microM of examined metals caused significant reduction of body bend and thermotaxis to cultivation temperature. Furthermore, Pb and Hg were the more toxic among the examined metals, with severe toxicity on associative learning behavior, thermotaxis, and locomotion behavior in nematodes.
在本研究中，使用趋热模型来评估不同浓度的金属暴露对线虫的联想学习行为的影响。检查的线虫在 25 或 17°C 下培养，然后转移到 20°C 条件下。基于线虫追踪 20°C 温度的能力，暴露于 10μM 的所有检查金属和 2.5μM 的 Pb 和 Hg 在 5 和 18 小时的时间间隔内显著降低了联想学习行为；然而，暴露于 2.5μM 的 Cu、Zn 和 Ag 并不影响联想学习行为。此外，暴露于 2.5 和 10μM 的检查金属并不影响体弯曲和对培养温度的趋热行为，而暴露于 50μM 的检查金属则显著降低了体弯曲和对培养温度的趋热行为。此外，Pb 和 Hg 是检查金属中毒性更强的金属，对线虫的联想学习行为、趋热行为和运动行为有严重毒性。
