低密度脂蛋白介导的DNA及DNA-抗DNA免疫复合物与细胞表面的相互作用。
LDL-mediated interaction of DNA and DNA-anti-DNA immune complexes with cell surface.
作者信息
Kabakov A E, Saenko V A, Poverenny A M
机构信息
Institute of Medical Radiology, Academy of Medical Sciences of the USSR, Obninsk.
出版信息
Clin Exp Immunol. 1991 Mar;83(3):359-63. doi: 10.1111/j.1365-2249.1991.tb05643.x.
The effect of low-density lipoprotein (LDL) on the binding of DNA and DNA-anti-DNA immune complexes to cultured human skin fibroblasts was examined. Using radioisotope analysis, ELISA and indirect immunofluorescent staining, a correlation between plasma membrane-bound DNA or DNA-anti-DNA immune complexes and cell-associated LDL was established. It was demonstrated that cytotoxicity and internalization of DNA-anti-DNA immune complexes may be LDL mediated. The data obtained suggest that the binding of the major part of DNA and immune complexes bound to surface of normal skin fibroblasts is due to the formation of a DNA-LDL-LDL receptor linkage. A possible role of LDL-containing immune complexes in the pathogenesis of systemic lupus erythematosus is discussed.
研究了低密度脂蛋白（LDL）对DNA及DNA-抗DNA免疫复合物与培养的人皮肤成纤维细胞结合的影响。采用放射性同位素分析、酶联免疫吸附测定（ELISA）及间接免疫荧光染色法，确定了细胞膜结合的DNA或DNA-抗DNA免疫复合物与细胞相关LDL之间的相关性。结果表明，DNA-抗DNA免疫复合物的细胞毒性和内化作用可能由LDL介导。所获数据提示，正常皮肤成纤维细胞表面结合的大部分DNA和免疫复合物的结合是由于形成了DNA-LDL-LDL受体连接。文中讨论了含LDL免疫复合物在系统性红斑狼疮发病机制中的可能作用。
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