Lamazière Antonin, Maniti Ofelia, Wolf Claude, Lambert Olivier, Chassaing Gérard, Trugnan Germain, Ayala-Sanmartin Jesus
Laboratoire des BioMolécules, Groupe N. J. Conté, Paris, France.
Biochim Biophys Acta. 2010 Dec;1798(12):2223-30. doi: 10.1016/j.bbamem.2009.12.024. Epub 2010 Jan 4.
Protein membrane transduction domains are able to translocate through cell membranes. This capacity resulted in new concepts on cell communication and in the design of vectors for internalization of active molecules into cells. Penetratin crosses the plasma membrane by a receptor and metabolic energy-independent mechanism which is at present unknown. A better knowledge of its interaction with phospholipids will help to understand the molecular mechanisms of cell penetration. Here, we investigated the role of lipid composition on penetratin induced membrane perturbations by X-ray diffraction, microscopy and (31)P-NMR. Penetratin showed the ability to induce phospholipid domain separation, membrane bilayer thickening, formation of vesicles, membrane undulations and tubular pearling. These data demonstrate its capacity to increase membrane curvature and suggest that dynamic phospholipid-penetratin complexes can be organized in different structural arrangements. These properties and their implications in peptide membrane translocation capacity are discussed.
蛋白质膜转导结构域能够穿过细胞膜。这种能力催生了关于细胞通讯的新概念，并推动了将活性分子内化到细胞中的载体设计。穿膜肽通过一种目前尚不清楚的受体和不依赖代谢能量的机制穿过质膜。深入了解其与磷脂的相互作用将有助于理解细胞穿透的分子机制。在此，我们通过X射线衍射、显微镜和（31）P-核磁共振研究了脂质组成对穿膜肽诱导的膜扰动的作用。穿膜肽显示出诱导磷脂结构域分离、膜双层增厚、囊泡形成、膜波动和管状珠化的能力。这些数据证明了其增加膜曲率的能力，并表明动态磷脂-穿膜肽复合物可以组织成不同的结构排列。本文讨论了这些特性及其对肽膜转位能力的影响。
