Department of Clinical and Experimental Medicine, Division of Clinical Chemistry, Linköping University, Linköping, Sweden.
Acta Biomater. 2010 Jul;6(7):2599-608. doi: 10.1016/j.actbio.2009.12.046. Epub 2010 Jan 4.
In this work, we have evaluated the haemocompatibility of different surface modifications, intended for biomaterials and bioanalytical applications. Polystyrene slides were coated with thin hydrogel films by self-initiated photografting and photopolymerization (SIPGP) of four different monomers. The hydrogel surface modifications were thoroughly characterized and tested for their protein resistance and ability to resist platelet adhesion and activation of the coagulation system. There was very little protein adsorption from human plasma on the hydrogels prepared from poly(ethylene glycol) methacrylate and 2-hydroxyethyl methacrylate. Platelet adhesion tests performed under both static and flow conditions showed that these coatings also demonstrated very high resistance towards platelet adhesion. A small amount of platelets were found to adhere to hydrogels formed from ethylene glycol methyl ether methacrylate and 2-carboxyethyl methacrylate. The polystyrene substrates themselves facilitated large amounts of platelet adhesion under both static and flow conditions. Utilizing a novel setup for imaging of coagulation, it was confirmed that none of the hydrogel surfaces activated the coagulation system to any great extent. We suggest that this simple fabrication method can be used to produce hydrogel coatings with unusually high blood compatibility, suitable for demanding biomaterials applications.
在这项工作中，我们评估了不同表面改性的血液相容性，旨在用于生物材料和生物分析应用。通过自引发光接枝和光聚合（SIPGP）聚合四种不同单体，将聚苯乙烯载玻片涂覆有薄的水凝胶膜。对水凝胶表面改性进行了彻底的表征，并测试了它们对蛋白质的抵抗力以及抵抗血小板黏附和凝血系统激活的能力。从人血浆中吸附到由聚乙二醇甲基丙烯酸酯和 2-羟乙基甲基丙烯酸酯制备的水凝胶上的蛋白质很少。在静态和流动条件下进行的血小板黏附试验表明，这些涂层对血小板黏附也具有很高的抵抗力。发现少量血小板黏附在由乙二醇甲基醚甲基丙烯酸酯和 2-羧乙基甲基丙烯酸酯形成的水凝胶上。聚苯乙烯基质本身在静态和流动条件下均有利于大量血小板黏附。利用用于凝血成像的新型装置，证实了没有一种水凝胶表面会很大程度上激活凝血系统。我们建议，这种简单的制造方法可用于生产血液相容性异常高的水凝胶涂层，适用于苛刻的生物材料应用。
