Key Laboratory of Terrestrial Ecological Processes, Institute of Applied Ecology, Shenyang 110116, PR China.
Environ Pollut. 2010 May;158(5):1530-5. doi: 10.1016/j.envpol.2009.12.024. Epub 2009 Dec 31.
This experiment examined the effects of sulfate (S) and reduced glutathione (GSH) on arsenic uptake by arsenic hyperaccumulator Pteris vittata after exposing to arsenate (0, 15 or 30 mg As L(-1)) with sulfate (6.4, 12.8 or 25.6 mg S L(-1)) or GSH (0, 0.4 or 0.8 mM) for 2-wk. Total arsenic, S and GSH concentrations in plant biomass and arsenic speciation in the growth media and plant biomass were determined. While both S (18-85%) and GSH (77-89%) significantly increased arsenic uptake in P. vittata, GSH also increased arsenic translocation by 61-85% at 0.4 mM (p < 0.05). Sulfate and GSH did not impact plant biomass or arsenic speciation in the media and biomass. The S-induced arsenic accumulation by P. vittata was partially attributed to increased plant GSH (21-31%), an important non-enzymatic antioxidant countering oxidative stress. This experiment demonstrated that S and GSH can effectively enhance arsenic uptake and translocation by P. vittata.
本实验研究了在暴露于砷酸盐（0、15 或 30mg As L(-1)）的条件下，硫酸盐（6.4、12.8 或 25.6mg S L(-1)）或还原型谷胱甘肽（GSH，0、0.4 或 0.8mM）对蜈蚣草（砷超积累植物）吸收砷的影响。测定了植物生物量中的总砷、S 和 GSH 浓度，以及生长介质和植物生物量中的砷形态。虽然 S（18-85%）和 GSH（77-89%）均显著增加了蜈蚣草对砷的吸收，但 0.4mM 的 GSH 还增加了 61-85%的砷转运（p<0.05）。硫酸盐和 GSH 对介质和生物量中的植物生物量或砷形态均没有影响。S 诱导的蜈蚣草砷积累部分归因于植物 GSH 的增加（21-31%），GSH 是一种重要的非酶抗氧化剂，可对抗氧化应激。本实验表明，S 和 GSH 可有效增强蜈蚣草对砷的吸收和转运。
