Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.
J Clin Neurosci. 2010 Feb;17(2):241-7. doi: 10.1016/j.jocn.2009.05.023. Epub 2009 Dec 31.
This study analyses aspects of the immune system in prolactinoma and non-secreting pituitary adenoma. Serum soluble major histocompatibility complex class I-related chain A protein (sMICA) concentrations were measured by enzyme-linked immunosorbent assay. NKG2D-expressing natural killer and T cells were analyzed by flow cytometry. A correlation analysis was also performed to associate sMICA levels with NKG2D expression. The expression of MICA was examined in specific tissues by use of the reverse transcription-polymerase chain reaction. A significant amount of sMICA was detected in the serum of nearly all patients. We found decreased percentage and mean fluorescence intensity of NKG2D-expressing natural killer and T cells from patients with prolactinoma and non-secreting pituitary adenoma compared to those from healthy donors. Pearson analysis showed a negative correlation between sMICA and NKG2D-expressing cells. The immune-escape of pituitary adenoma is related to the down-regulation of NKG2D and the up-regulation of its ligand MICA.
本研究分析了催乳素瘤和无分泌性垂体腺瘤中免疫系统的各个方面。通过酶联免疫吸附试验测量了血清可溶性主要组织相容性复合体 I 类相关链 A 蛋白 (sMICA) 的浓度。通过流式细胞术分析了表达 NKG2D 的自然杀伤细胞和 T 细胞。还进行了相关性分析，以将 sMICA 水平与 NKG2D 表达相关联。使用逆转录聚合酶链反应检查了 MICA 在特定组织中的表达。几乎所有患者的血清中都检测到大量的 sMICA。我们发现，与健康供体相比，催乳素瘤和无分泌性垂体腺瘤患者表达 NKG2D 的自然杀伤细胞和 T 细胞的百分比和平均荧光强度降低。皮尔逊分析显示 sMICA 与表达 NKG2D 的细胞之间呈负相关。垂体腺瘤的免疫逃避与 NKG2D 的下调和其配体 MICA 的上调有关。
