IGF-1 对小鼠 11β-羟类固醇脱氢酶 1 和 2 的调节作用。
Regulation of 11beta-hydroxysteroid dehydrogenase 1 and 2 by IGF-1 in mice.
机构信息
Institutes of Life and Health Engineering, Jinan University, Guangzhou, Guangdong 510632, PR China.
出版信息
Biochem Biophys Res Commun. 2010 Jan 22;391(4):1752-6. doi: 10.1016/j.bbrc.2009.12.148. Epub 2010 Jan 1.
Two isoforms of 11beta-hydroxysteroid dehydrogenase (11beta-HSD1 and 11beta-HSD2) play an important role in regulation of glucocorticoid corticosterone (CORT, the active form in rodents) by the interconversion between CORT and 11-dehydrocorticosterone (11DHC, the biologically inert form). 11beta-HSD1 is an NADP+/NADPH-dependent oxidoreductase which is mainly expressed in liver and kidney, while 11beta-HSD2 is an NAD+-dependent oxidase which is predominantly expressed in kidney. The regulation of 11beta-HSD1 and 11beta-HSD2 mRNA (Hsd11b1 and Hsd11b2) levels and their activities by IGF-1 was performed in liver, kidney, and testis of IGF-1 knockout male mice. Real-time PCR showed that Hsd11b1 in liver was decreased while Hsd11b2 mRNA level was decreased in kidney of IGF-1 null mice. 11beta-HSD1 and 11beta-HSD2 activities fluctuated with the changes of their respective Hsd11b1 or Hsd11b2 mRNA levels. In conclusion, IGF-I tissue-specifically regulates Hsd11b1 and Hsd11b2 expression.
两种 11β-羟类固醇脱氢酶（11β-HSD1 和 11β-HSD2）同工酶在糖皮质激素皮质酮（CORT，啮齿动物中的活性形式）和 11-脱氢皮质酮（11DHC，生物惰性形式）的相互转化中对糖皮质激素的调节中起着重要作用。11β-HSD1 是一种 NADP+/NADPH 依赖性氧化还原酶，主要在肝脏和肾脏中表达，而 11β-HSD2 是一种 NAD+-依赖性氧化酶，主要在肾脏中表达。通过 IGF-1 在 IGF-1 敲除雄性小鼠的肝脏、肾脏和睾丸中对 11β-HSD1 和 11β-HSD2 mRNA（Hsd11b1 和 Hsd11b2）水平及其活性进行了调节。实时 PCR 显示，IGF-1 缺失小鼠肝脏中的 Hsd11b1 减少，而肾脏中的 Hsd11b2 mRNA 水平降低。11β-HSD1 和 11β-HSD2 的活性随其各自的 Hsd11b1 或 Hsd11b2 mRNA 水平的变化而波动。总之，IGF-I 组织特异性地调节 Hsd11b1 和 Hsd11b2 的表达。
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